Proteomic characterization of the fibroin-based silk fibers produced by weaver ant Camponotus textor

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorCenter for Genetic Engineering and Biotechnology (CIGB)-
Autor(es): dc.creatorPinto, José Roberto Aparecido dos Santos-
Autor(es): dc.creatorEsteves, Franciele Grego-
Autor(es): dc.creatorTormena, Cláudio Francisco-
Autor(es): dc.creatorPerez-Riverol, Amilcar-
Autor(es): dc.creatorLasa, Alexis Musacchio-
Autor(es): dc.creatorBueno, Odair Correa-
Autor(es): dc.creatorPalma, Mario Sergio-
Data de aceite: dc.date.accessioned2025-08-21T22:01:00Z-
Data de disponibilização: dc.date.available2025-08-21T22:01:00Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-06-15-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jprot.2022.104579-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/240857-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/240857-
Descrição: dc.descriptionThe fibroin-based silk fibers of weaver ants are an alternative biomaterial to be investigated and explored for potential biomedical applications. In this context, the silk fibers from the nest of the weaver ant Camponotus textor was solubilized and fractionated by gel permeation. The different fractions were collected, pooled and submitted to analysis with a series of biochemical methods, nuclear magnetic resonance (NMR) spectroscopy, analytical proteomic strategies, and data treatment with bioinformatic tools to perform the structural characterization of the fibroin-based silk fibers produced by the ant. Our data demonstrated the identification of one fibroin proteoform in the ant silk fibers. The protein chracterized as a glycoprotein with MW around 40 kDa and presenting 66% (w/w) of total sugars attached to it through O-linked carbohydrates. The 3D of protein was modeled, revealing a structure predominantly constituted of coiled-coil secondary units in the whole model, featuring at least four superhelices (arrangement with multiple α-helices). The scientific outcomes reported herein may be relevant for the development of novel approaches for the synthetic or recombinant production of novel silk-based polymers for biomedical applications. Biological significance: The present investigation significantly expanded knowledge regarding to the fibroin-based silk fibers from weaver ants, contributing to improvements in our understanding of the properties and characteristics of these silk fibers. For example, as reported here, carbohydrates were detected in the ants' silk for the first time presenting the fibroin as a glycoprotein. Moreover, the 3D structure provided new insights into the secondary structures considering the whole model of the protein.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of General and Applied Biology Institute of Biosciences of Rio Claro University of Sao Paulo State (UNESP), Rio Claro-
Descrição: dc.descriptionInstitute of Chemistry University of Campinas (UNICAMP), Campinas-
Descrição: dc.descriptionBiomedical Research Division Center for Genetic Engineering and Biotechnology (CIGB)-
Descrição: dc.descriptionDepartment of General and Applied Biology Institute of Biosciences of Rio Claro University of Sao Paulo State (UNESP), Rio Claro-
Idioma: dc.languageen-
Relação: dc.relationJournal of Proteomics-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAnt silk proteins-
Palavras-chave: dc.subjectCarbohydrates-
Palavras-chave: dc.subjectFibroins-
Palavras-chave: dc.subjectLCMS-based proteomics-
Palavras-chave: dc.subjectNMR-
Título: dc.titleProteomic characterization of the fibroin-based silk fibers produced by weaver ant Camponotus textor-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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