Bothrops leucurus snake venom protein profile, isolation and biological characterization of its major toxin PLA2s-like

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Autor(es): dc.contributorState University of Feira de Santana (UEFS)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal da Bahia (UFBA)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorState University of Feira de Santana-
Autor(es): dc.contributorButantan Institute-
Autor(es): dc.creatorDias, Êuder Reis-
Autor(es): dc.creatorOliveira, Laudicéia Alves de-
Autor(es): dc.creatorLauria, Pedro Santana Sales-
Autor(es): dc.creatorBordon, Karla de Castro Figueiredo-
Autor(es): dc.creatorDomênico, Adriana Moraes Rodrigues-
Autor(es): dc.creatorGuerreiro, Marcos Lázaro da Silva-
Autor(es): dc.creatorWiezel, Gisele Adriano-
Autor(es): dc.creatorCardoso, Iara Aimê-
Autor(es): dc.creatorRossini, Bruno César-
Autor(es): dc.creatorMarino, Celso Luís-
Autor(es): dc.creatorPimenta, Daniel Carvalho-
Autor(es): dc.creatorArantes, Eliane Candiani-
Autor(es): dc.creatorCasais-e-Silva, Luciana Lyra-
Autor(es): dc.creatorBranco, Alexsandro-
Autor(es): dc.creatorSantos, Lucilene Delazari dos-
Autor(es): dc.creatorBiondi, Ilka-
Data de aceite: dc.date.accessioned2025-08-21T22:29:54Z-
Data de disponibilização: dc.date.available2025-08-21T22:29:54Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-07-15-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.toxicon.2022.04.002-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/240843-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/240843-
Descrição: dc.descriptionBothrops leucurus is considered as a snake of medical interest in the State of Bahia, Brazil. However, so far, there are no studies that provide a refined mapping of the composition of this venom. The aim of this work was to better understand the protein composition of B. leucurus snake venom and to isolate and biologically characterize the most abundant toxin, a basic PLA2-like. Shotgun proteomics approach identified 137 protein hits in B. leucurus venom subdivided into 19 protein families. The new basic PLA2-like toxin identified was denominated Bleu-PLA2-like, it and other proteoforms represents about 25% of the total proteins in the venom of B. leucurus and induces myotoxicity, inflammation and muscle damage. Immunoreactivity assays demonstrated that B. leucurus venom is moderately recognized by bothropic and crotalic antivenoms, and on the other hand, Bleu-PLA2-like and its proteoforms are poorly recognized. Our findings open doors for future studies in order to assess the systemic effects caused by this snake venom in order to better understand the toxinological implications of this envenomation and, consequently, to assist in the clinical treatment of victims.-
Descrição: dc.descriptionUniversidade Estadual Paulista-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado da Bahia-
Descrição: dc.descriptionGraduate Program in Biotechnology State University of Feira de Santana (UEFS), BA-
Descrição: dc.descriptionGraduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP), SP-
Descrição: dc.descriptionLaboratory of Pharmacology and Experimental Therapeutics College of Pharmacy Federal University of Bahia, BA-
Descrição: dc.descriptionDepartment of BioMolecular Sciences School of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo (USP), SP-
Descrição: dc.descriptionSpecialization in Diagnostic and Therapeutic Innovations Hospital das Clínicas São Paulo State University (UNESP), SP-
Descrição: dc.descriptionLaboratory of Venomous Animals and Herpetology State University of Feira de Santana, BA-
Descrição: dc.descriptionBiotechnology Institute (IBTEC) São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Biological and Chemical Sciences São Paulo State University (UNESP), SP-
Descrição: dc.descriptionLaboratory of Biochemistry and Biophysics Butantan Institute, SP-
Descrição: dc.descriptionLaboratory of Neuroimmunoendocrinology and Toxinology Institute of Health Sciences Federal University of Bahia, BA-
Descrição: dc.descriptionHealth Department State University of Feira de Santana, BA-
Descrição: dc.descriptionGraduate Program in Graduate Program in Research and Development (Medical Biotechnology) Botucatu Medical School (FMB) São Paulo State University (UNESP), SP-
Descrição: dc.descriptionGraduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP), SP-
Descrição: dc.descriptionSpecialization in Diagnostic and Therapeutic Innovations Hospital das Clínicas São Paulo State University (UNESP), SP-
Descrição: dc.descriptionBiotechnology Institute (IBTEC) São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Biological and Chemical Sciences São Paulo State University (UNESP), SP-
Descrição: dc.descriptionGraduate Program in Graduate Program in Research and Development (Medical Biotechnology) Botucatu Medical School (FMB) São Paulo State University (UNESP), SP-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2017/00586–6-
Descrição: dc.descriptionCNPq: 315026/2021–9-
Descrição: dc.descriptionCNPq: 406385/2018–1-
Descrição: dc.descriptionCNPq: 437089/2018–5-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado da Bahia: FAPESB-DTE0014/2015-
Formato: dc.format27-42-
Idioma: dc.languageen-
Relação: dc.relationToxicon-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBothrops leucurus-
Palavras-chave: dc.subjectMass spectrometry-
Palavras-chave: dc.subjectPhospholipase A2-
Palavras-chave: dc.subjectProteome-
Palavras-chave: dc.subjectSnake venom-
Título: dc.titleBothrops leucurus snake venom protein profile, isolation and biological characterization of its major toxin PLA2s-like-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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