SEQUENCE SLIDER: integration of structural and genetic data to characterize isoforms from natural sources

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorInstitute of Molecular Biology of Barcelona (IBMB-CSIC)-
Autor(es): dc.contributorICREA-
Autor(es): dc.creatorBorges, Rafael J.-
Autor(es): dc.creatorSalvador, Guilherme H M-
Autor(es): dc.creatorPimenta, Daniel C.-
Autor(es): dc.creatorSantos, Lucilene D. dos-
Autor(es): dc.creatorFontes, Marcos R M-
Autor(es): dc.creatorUsón, Isabel-
Data de aceite: dc.date.accessioned2025-08-21T19:50:46Z-
Data de disponibilização: dc.date.available2025-08-21T19:50:46Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-05-20-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1093/nar/gkac029-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/240818-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/240818-
Descrição: dc.descriptionProteins isolated from natural sources can be composed of a mixture of isoforms with similar physicochemical properties that coexist in the final steps of purification. Yet, even where unverified, the assumed sequence is enforced throughout the structural studies. Herein, we propose a novel perspective to address the usually neglected sequence heterogeneity of natural products by integrating biophysical, genetic and structural data in our program SEQUENCE SLIDER. The aim is to assess the evidence supporting chemical composition in structure determination. Locally, we interrogate the experimental map to establish which side chains are supported by the structural data, and the genetic information relating sequence conservation is integrated into this statistic. Hence, we build a constrained peptide database, containing most probable sequences to interpret mass spectrometry data (MS). In parallel, we perform MS de novo sequencing with genomic-based algorithms to detect point mutations. We calibrated SLIDER with Gallus gallus lysozyme, whose sequence is unequivocally established and numerous natural isoforms are reported. We used SLIDER to characterize a metalloproteinase and a phospholipase A2-like protein from the venom of Bothrops moojeni and a crotoxin from Crotalus durissus collilineatus. This integrated approach offers a more realistic structural descriptor to characterize macromolecules isolated from natural sources.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionMinisterio de Ciencia e Innovación-
Descrição: dc.descriptionDepartament of Biophysics and Pharmacology Biosciences Institute São Paulo State University (UNESP)-
Descrição: dc.descriptionCrystallographic Methods Institute of Molecular Biology of Barcelona (IBMB-CSIC)-
Descrição: dc.descriptionBiochemistry and Biophysics Laboratory, Butantan Institute, São Paulo, São Paulo 05503-900, Brazil-
Descrição: dc.descriptionGraduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP)-
Descrição: dc.descriptionBiotechnology Institute (IBTEC) São Paulo State University (UNESP)-
Descrição: dc.descriptionICREA, Pg. Lluís Companys 23-
Descrição: dc.descriptionDepartament of Biophysics and Pharmacology Biosciences Institute São Paulo State University (UNESP)-
Descrição: dc.descriptionGraduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP)-
Descrição: dc.descriptionBiotechnology Institute (IBTEC) São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2015/17286-0-
Descrição: dc.descriptionFAPESP: 2016/24191-8-
Descrição: dc.descriptionCNPq: 301974/2019-5-
Descrição: dc.descriptionMinisterio de Ciencia e Innovación: PGC2018-101370-BI00 AEI/FEDER/UE-
Formato: dc.formate50-
Idioma: dc.languageen-
Relação: dc.relationNucleic acids research-
???dc.source???: dc.sourceScopus-
Título: dc.titleSEQUENCE SLIDER: integration of structural and genetic data to characterize isoforms from natural sources-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.