PTH 1-34-functionalized bioactive glass improves peri-implant bone repair in orchiectomized rats: Microscale and ultrastructural evaluation

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorMcMaster University-
Autor(es): dc.creatorGomes-Ferreira, Pedro Henrique Silva-
Autor(es): dc.creatorMicheletti, Chiara-
Autor(es): dc.creatorFrigério, Paula Buzo-
Autor(es): dc.creatorBatista, Fábio Roberto de Souza-
Autor(es): dc.creatorMonteiro, Naara Gabriela-
Autor(es): dc.creatorBim-júnior, Odair-
Autor(es): dc.creatorLisboa-Filho, Paulo Noronha-
Autor(es): dc.creatorGrandfield, Kathryn-
Autor(es): dc.creatorOkamoto, Roberta-
Data de aceite: dc.date.accessioned2025-08-21T16:19:23Z-
Data de disponibilização: dc.date.available2025-08-21T16:19:23Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.msec.2022.112688-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/240817-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/240817-
Descrição: dc.descriptionThe objective of this work was to investigate the use of Biogran® functionalized with parathyroid hormone (PTH) 1–34 by sonochemistry for the local delivery of this anabolic agent to the implant site. The effects of Biogran® and topical administration of PTH 1–34 on peri-implant bone regeneration were evaluated from the microscale to ultrastructural levels in healthy (SHAM) and orchiectomized (ORQ). While some animals only received a titanium implant in their tibial metaphyses (CLOT group), in others the peri-implant defect was first filled with Biogran® either without or with PTH 1–34 functionalization (BG and BGPTH groups, respectively) prior to implant installation. Osseointegration was characterized from a biomechanical perspective by measuring the removal torque with the counter-torque technique. Micro-CT was used to evaluate the percentage of bone volume, trabecular thickness, number and separation, and bone-implant contact (BIC). Dynamics of new bone formation were assessed by measuring fluorochrome area, daily mineral apposition rate, and neoformed bone area using confocal laser microscopy. RT-PCR was performed to evaluate ALP and osteocalcin expression. The interface between newly formed bone and Biogran® was examined using scanning electron microscopy (SEM) and scanning transmission electron microscopy (STEM) at the micro-and nanoscale, respectively, while elemental analyses were completed in SEM with energy-dispersive X-ray spectroscopy (EDS). STEM imaging demonstrated the intimate attachment of bone to Biogran® (nanoscale level). Overall, the results suggest that the effectiveness of the topical administration of PTH 1–34 at the implant site seems enhanced in osteoporotic bone, promoting peri-implant bone regeneration to comparable levels in healthy conditions.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionUniversidade Estadual Paulista-
Descrição: dc.descriptionFinanciadora de Estudos e Projetos-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Surgery and Integrated Clinic São Paulo State University Araçatuba Dental School, SP-
Descrição: dc.descriptionDepartment of Materials Science and Engineering McMaster University-
Descrição: dc.descriptionSão Paulo State University Araçatuba Dental School, SP-
Descrição: dc.descriptionDepartment of Physics São Paulo State University School of Sciences, SP-
Descrição: dc.descriptionSchool of Biomedical Engineering McMaster University-
Descrição: dc.descriptionDepartment of Surgery and Integrated Clinic São Paulo State University Araçatuba Dental School, SP-
Descrição: dc.descriptionSão Paulo State University Araçatuba Dental School, SP-
Descrição: dc.descriptionDepartment of Physics São Paulo State University School of Sciences, SP-
Descrição: dc.descriptionFinanciadora de Estudos e Projetos: 01.12.0530.00 PROINFRA 01/2011-
Descrição: dc.descriptionFAPESP: 2015/14688-0-
Descrição: dc.descriptionFAPESP: 2017/08187-3-
Idioma: dc.languageen-
Relação: dc.relationBiomaterials Advances-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBioactive glass-
Palavras-chave: dc.subjectBone regeneration-
Palavras-chave: dc.subjectDental implants-
Palavras-chave: dc.subjectLocal drug delivery-
Palavras-chave: dc.subjectOsteoporosis-
Palavras-chave: dc.subjectParathyroid hormone-
Título: dc.titlePTH 1-34-functionalized bioactive glass improves peri-implant bone repair in orchiectomized rats: Microscale and ultrastructural evaluation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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