Protein Profiling of a Cellular Model of NAFLD by Advanced Bioanalytical Approaches

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Autor(es): dc.contributorUniversity of Milan-
Autor(es): dc.contributorTelematic University San Raffaele-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorAltomare, Alessandra Anna-
Autor(es): dc.creatorAiello, Gilda-
Autor(es): dc.creatorGarcia, Jessica Leite-
Autor(es): dc.creatorGarrone, Giulia-
Autor(es): dc.creatorZoanni, Beatrice-
Autor(es): dc.creatorCarini, Marina-
Autor(es): dc.creatorAldini, Giancarlo-
Autor(es): dc.creatorD’Amato, Alfonsina-
Data de aceite: dc.date.accessioned2025-08-21T20:06:01Z-
Data de disponibilização: dc.date.available2025-08-21T20:06:01Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ijms23169025-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/240705-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/240705-
Descrição: dc.descriptionAdvanced quantitative bioanalytical approaches in combination with network analyses allow us to answer complex biological questions, such as the description of changes in protein profiles under disease conditions or upon treatment with drugs. In the present work, three quantitative proteomic approaches—either based on labelling or not—in combination with network analyses were applied to a new in vitro cellular model of nonalcoholic fatty liver disease (NAFLD) for the first time. This disease is characterized by the accumulation of lipids, inflammation, fibrosis, and insulin resistance. Hepatic G2 cells were used as model, and NAFLD was induced by a complex of oleic acid and bovine albumin. The development of the disease was verified by lipid vesicle staining and by the increase in the expression of perilipin-2—a protein constitutively present in the vesicles during NAFLD. The nLC–MS/MS analyses of peptide samples obtained from three different proteomic approaches resulted in accurate and reproducible quantitative data of protein fold-change expressed in NAFLD versus control cells. The differentially regulated proteins were used to evaluate the involved and statistically enriched pathways. Network analyses highlighted several functional and disease modules affected by NAFLD, such as inflammation, oxidative stress defense, cell proliferation, and ferroptosis. Each quantitative approach allowed the identification of similar modulated pathways. The combination of the three approaches improved the power of statistical network analyses by increasing the number of involved proteins and their fold-change. In conclusion, the application of advanced bioanalytical approaches in combination with pathway analyses allows the in-depth and accurate description of the protein profile of an in vitro cellular model of NAFLD by using high-resolution quantitative mass spectrometry data. This model could be extremely useful in the discovery of new drugs to modulate the equilibrium NAFLD health state.-
Descrição: dc.descriptionDepartment of Pharmaceutical Sciences University of Milan, Via L. Mangiagalli 25-
Descrição: dc.descriptionDepartment of Human Science and Quality of Life Promotion Telematic University San Raffaele-
Descrição: dc.descriptionMedical School Sao Paulo State University-
Descrição: dc.descriptionUnitech OMICs Platform University of Milan, Viale Ortles 22/4-
Descrição: dc.descriptionMedical School Sao Paulo State University-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Molecular Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectNAFLD-
Palavras-chave: dc.subjectnano liquid chromatography-
Palavras-chave: dc.subjectproteins-
Palavras-chave: dc.subjectsignaling-
Palavras-chave: dc.subjecttandem mass spectrometry-
Título: dc.titleProtein Profiling of a Cellular Model of NAFLD by Advanced Bioanalytical Approaches-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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