Mercury metalloproteomic profile in muscle tissue of Arapaima gigas from the Brazilian Amazon

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Nebraska (UNL)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversity of Brasília (UNB)-
Autor(es): dc.creatorBataglioli, Izabela da Cunha-
Autor(es): dc.creatorQueiroz, João Vitor de-
Autor(es): dc.creatorVieira, José Cavalcante Souza-
Autor(es): dc.creatorCavalline, Nubya Gonçalves-
Autor(es): dc.creatorBraga, Camila Pereira-
Autor(es): dc.creatorBuzalaf, Marília Afonso Rabelo-
Autor(es): dc.creatorZara, Luís Fabrício-
Autor(es): dc.creatorAdamec, Jiri-
Autor(es): dc.creatorPadilha, Pedro de Magalhães[UNESP]-
Data de aceite: dc.date.accessioned2025-08-21T17:39:15Z-
Data de disponibilização: dc.date.available2025-08-21T17:39:15Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s10661-022-10357-5-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/240692-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/240692-
Descrição: dc.descriptionMetalloproteomics is an innovative methodology for identifying of protein-associated mercury. Thus, we analyzed the muscle proteome of Arapaima gigas (pirarucu), collected in the Madeira River of the Brazilian Amazon, to identify protein-associated mercury, with the aim of identifying possible mercury biomarkers in fish muscle tissue. After obtaining the protein pellet, we conducted two-dimensional electrophoresis (2D PAGE) to fractionate the muscle proteome. Total mercury in muscle tissue and protein pellets and mapping of mercury content in protein spots of the 2D PAGE gels was determined using graphite furnace atomic absorption spectrometry (GFAAS). The protein-associated mercury identification was performed using liquid chromatography coupled with sequence mass spectrometry (LC‒MS/MS). Total mercury determinations by GFAAS indicated concentrations on the order of 153 ± 1.90 mg kg−1 and 142 ± 1.50 mg kg−1 (total precipitation of protein fraction) and 139 ± 1.45 mg kg−1 (fractional precipitation of protein fraction) in muscle tissue and protein pellets, respectively. Mercury concentrations in the range of 48 ± 0.90 to 165 ± 3.00 mg kg−1 were found in twelve protein spots. Among the 2D PAGE protein spots, eleven Hg-binding proteins were identified using LC‒MS/MS, which showed characteristics of mercury exposure biomarkers for important metabolic functions, such as five parvalbumin isoforms, triosephosphate isomerase, cofilin 2 (muscle), and fructose-bisphosphate aldolases.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionSchool of Veterinary Medicine and Animal Science São Paulo State University (UNESP)-
Descrição: dc.descriptionInstitute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionUniversity of Nebraska (UNL)-
Descrição: dc.descriptionUniversity of São Paulo (USP)-
Descrição: dc.descriptionCollege of Planaltina University of Brasília (UNB)-
Descrição: dc.descriptionSchool of Veterinary Medicine and Animal Science São Paulo State University (UNESP)-
Descrição: dc.descriptionInstitute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionCAPES: 1-
Descrição: dc.descriptionFAPESP: 1-
Descrição: dc.descriptionFAPESP: 2-
Descrição: dc.descriptionCNPq: 3-
Idioma: dc.languageen-
Relação: dc.relationEnvironmental Monitoring and Assessment-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAmazon fish-
Palavras-chave: dc.subjectHg-binding proteins-
Palavras-chave: dc.subjectMercury-
Palavras-chave: dc.subjectMercury metallomics study-
Título: dc.titleMercury metalloproteomic profile in muscle tissue of Arapaima gigas from the Brazilian Amazon-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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