Anticancer activities of Brachydin C in human prostate tumor cells (DU145) grown in 2D and 3D models: Stimulation of cell death and downregulation of metalloproteinases in spheroids

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual de Londrina (UEL)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFederal University of Maranhão-
Autor(es): dc.creatorOliveira, Larissa Cristina Bastos de-
Autor(es): dc.creatorRibeiro, Diego Luis-
Autor(es): dc.creatorNascimento, Jessyane Rodrigues do-
Autor(es): dc.creatorRocha, Claudia Quintino da-
Autor(es): dc.creatorCólus, Ilce Mara de Syllos-
Autor(es): dc.creatorSerpeloni, Juliana Mara-
Data de aceite: dc.date.accessioned2025-08-21T21:51:12Z-
Data de disponibilização: dc.date.available2025-08-21T21:51:12Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/cbdd.14112-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/240495-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/240495-
Descrição: dc.descriptionBrachydin C (BrC) has demonstrated in vitro cytotoxic and antiproliferative effects in prostate cancer cells. In the present study, we compare the anticancer effects of BrC in DU145 cells grown in common bidimensional cultures (2D) and multicellular tumor spheroids (MCTS), often denominated 3D in vitro models, that can better mimic the microenvironment of tissues. BrC IC50 values obtained in the resazurin assay after 24 h of treatment were 47.31 μM (2D) and 229.8 μM (3D) and these cytotoxic effects were time-dependent only in 3D. BrC (5.0–60 μM) interfered with the growth of MCTS and reduced cell viability after 11 days of treatment, a result that is not attributable to oxidative stress evaluated using the CM-H2DCFDA probe. BrC (6.0 μM) impaired horizontal (wound-healing) and vertical cell migration and invasion (transwell assay) in 2D and BrC (5.0–60 μM) in 3D (ECM Gel®). BrC modulated the expression of genes BIRC5, TNF-α, CASP3, NKX3.1, MMP9, MMP11, CDH1, and ITGAM and downregulated proteins CASP7, BAX, and TNF-α in Western blotting analysis. In conclusion, BrC stimulated cell death and decreased epithelial–mesenchymal transition. Furthermore, DU145 MCTS displayed higher resistance to BrC-induced cell death than 2D cultures, a difference that should be considered in future approaches in prostatic cancer studies.-
Descrição: dc.descriptionDepartment of General Biology Center of Biological Sciences State University of Londrina (UEL)-
Descrição: dc.descriptionDepartment of Genetics Ribeirão Preto Medical School University of São Paulo (USP)-
Descrição: dc.descriptionChemistry Institute São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Chemistry Center for Exact Sciences and Technology Federal University of Maranhão-
Descrição: dc.descriptionChemistry Institute São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationChemical Biology and Drug Design-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectchemotherapeutic-
Palavras-chave: dc.subjectFridericia platyphylla-
Palavras-chave: dc.subjectmetalloproteinases-
Palavras-chave: dc.subjectphytochemical-
Palavras-chave: dc.subjectsurvivin-
Título: dc.titleAnticancer activities of Brachydin C in human prostate tumor cells (DU145) grown in 2D and 3D models: Stimulation of cell death and downregulation of metalloproteinases in spheroids-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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