Melatonin Reverses the Warburg-Type Metabolism and Reduces Mitochondrial Membrane Potential of Ovarian Cancer Cells Independent of MT1 Receptor Activation

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFAMERP-
Autor(es): dc.contributorUniversidade Estadual do Norte do Paraná—UENP-
Autor(es): dc.contributorUT Health-
Autor(es): dc.creatorCucielo, Maira Smaniotto-
Autor(es): dc.creatorCesário, Roberta Carvalho-
Autor(es): dc.creatorSilveira, Henrique Spaulonci-
Autor(es): dc.creatorGaiotte, Letícia Barbosa-
Autor(es): dc.creatorSantos, Sérgio Alexandre Alcantara dos-
Autor(es): dc.creatorZuccari, Debora Aparecida Pires de Campos-
Autor(es): dc.creatorSeiva, Fábio Rodrigues Ferreira-
Autor(es): dc.creatorReiter, Russel J.-
Autor(es): dc.creatorChuffa, Luiz Gustavo de Almeida-
Data de aceite: dc.date.accessioned2025-08-21T21:15:25Z-
Data de disponibilização: dc.date.available2025-08-21T21:15:25Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/molecules27144350-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/240427-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/240427-
Descrição: dc.descriptionOvarian cancer (OC) is the most lethal gynecologic malignancy, and melatonin has shown various antitumor properties. Herein, we investigated the influence of melatonin therapy on energy metabolism and mitochondrial integrity in SKOV-3 cells and tested whether its effects depended on MT1 receptor activation. SKOV-3 cells were exposed to different melatonin concentrations, and experimental groups were divided as to the presence of MT1 receptors (melatonin groups) or receptor absence by RNAi silencing (siRNA MT1+melatonin). Intracellular melatonin levels increased after treatment with melatonin independent of the MT1. The mitochondrial membrane potential of SKOV-3 cells decreased in the group treated with the highest melatonin concentration. Melatonin reduced cellular glucose consumption, while MT1 knockdown increased its consumption. Interconversion of lactate to pyruvate increased after treatment with melatonin and was remarkable in siRNA MT1 groups. Moreover, lactate dehydrogenase activity decreased with melatonin and increased after MT1 silencing at all concentrations. The UCSC XenaBrowser tool showed a positive correlation between the human ASMTL gene and the ATP synthase genes, succinate dehydrogenase gene (SDHD), and pyruvate dehydrogenase genes (PDHA and PDHB). We conclude that melatonin changes the glycolytic phenotype and mitochondrial integrity of SKOV-3 cells independent of the MT1 receptor, thus decreasing the survival advantage of OC cells.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences UNESP—São Paulo State University, São Paulo-
Descrição: dc.descriptionCancer Molecular Research Laboratory (LIMC) FAMERP Department of Molecular Biology, São Paulo-
Descrição: dc.descriptionBiological Science Center Department of Biology Luiz Meneghel Campus Universidade Estadual do Norte do Paraná—UENP, Paraná-
Descrição: dc.descriptionDepartment of Cell Systems and Anatomy UT Health-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences UNESP—São Paulo State University, São Paulo-
Descrição: dc.descriptionFAPESP: 2018/15797-5-
Descrição: dc.descriptionFAPESP: 2019/00906-6-
Idioma: dc.languageen-
Relação: dc.relationMolecules-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectglucose-
Palavras-chave: dc.subjectmelatonin-
Palavras-chave: dc.subjectmitochondrial metabolism-
Palavras-chave: dc.subjectovarian cancer-
Palavras-chave: dc.subjectSKOV-3 cells-
Palavras-chave: dc.subjectWarburg effect-
Título: dc.titleMelatonin Reverses the Warburg-Type Metabolism and Reduces Mitochondrial Membrane Potential of Ovarian Cancer Cells Independent of MT1 Receptor Activation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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