Unusual dimeric flavonoids (brachydins) induce ultrastructural membrane alterations associated with antitumor activity in cancer cell lines

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorFederal University of Maranhão-
Autor(es): dc.contributorState University of Maranhão-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorMaciel-Silva, Vera Lucia-
Autor(es): dc.creatorRocha, Claudia Quintino da-
Autor(es): dc.creatorAlencar, Luciana Magalhães Rebelo-
Autor(es): dc.creatorCastelo-Branco, Patrícia Valéria-
Autor(es): dc.creatorSousa, Israel Higino de-
Autor(es): dc.creatorAzevedo-Santos, Ana Paula-
Autor(es): dc.creatorVale, André Alvares Marques-
Autor(es): dc.creatorMonteiro, Silvio Gomes-
Autor(es): dc.creatorSoares, Rossy-Eric Pereira-
Autor(es): dc.creatorGuimarães, Sulayne Janayna Araujo-
Autor(es): dc.creatorNascimento, Jessyane Rodrigues do-
Autor(es): dc.creatorPereira, Silma Regina Ferreira-
Data de aceite: dc.date.accessioned2025-08-21T19:20:58Z-
Data de disponibilização: dc.date.available2025-08-21T19:20:58Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1080/01480545.2022.2080217-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/240214-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/240214-
Descrição: dc.descriptionNotwithstanding the advances in molecular target-based drugs, chemotherapy remains the most common cancer treatment, despite its high toxicity. Consequently, effective anticancer therapies with fewer adverse effects are needed. Therefore, this study aimed to determine the anticancer activity of the dichloromethane fraction (DCMF) isolated from Arrabidae brachypoda roots, whose components are three unusual dimeric flavonoids. The toxicity of DCMF was investigated in breast (MCF-7), prostate (DU145), and cervical (HeLa) tumor cells, as well as non-tumor cells (PNT2), using sulforhodamine B (cell viability), Comet (genotoxicity), clonogenicity (reproductive capacity) and wound healing (cell migration) assays, and atomic force microscopy (AFM) for ultrastructural cell membrane alterations. Molecular docking revealed affinity between albumin and each rare flavonoid, supporting the impact of fetal bovine serum in DCMF antitumor activity. The IC50 values for MCF7, HeLa, and DU145 were 2.77, 2.46, and 2.51 µg/mL, respectively, and 4.08 µg/mL for PNT2. DCFM was not genotoxic to tumor or normal cells when exposed to twice the IC50 for up to 24 h, but it inhibited tumor cell migration and reproduction compared to normal cells. Additionally, AFM revealed alterations in the ultrastructure of tumor nuclear membrane surfaces, with a positive correlation between DCMF concentration and tumor cell roughness. Finally, we found a negative correlation between roughness and the ability of DCMF-treated tumor cells to migrate and form colonies with more than 50 cells. These findings suggest that DCFM acts by causing ultrastructural changes in tumor cell membranes while having fewer toxicological effects on normal cells.-
Descrição: dc.descriptionPostgraduate Program in Biodiversity and Biotechnology-Bionorte Federal University of Maranhão-
Descrição: dc.descriptionLaboratory of Genetics and Molecular Biology Department of Biology Federal University of Maranhão-
Descrição: dc.descriptionDepartment of Biology State University of Maranhão-
Descrição: dc.descriptionLaboratory of Natural Products Department of Chemistry Federal University of Maranhão-
Descrição: dc.descriptionLaboratory of Biophysics and Nanosystems Department of Physics Federal University of Maranhão-
Descrição: dc.descriptionLaboratory of Immunology Applied to Cancer Department of Physiological Sciences Federal University of Maranhão-
Descrição: dc.descriptionPostgraduate Program in Health Sciences Federal University of Maranhão-
Descrição: dc.descriptionPostgraduate Program in Chemistry São Paulo State University (Unesp) Institute of Chemistry-
Descrição: dc.descriptionPostgraduate Program in Chemistry São Paulo State University (Unesp) Institute of Chemistry-
Idioma: dc.languageen-
Relação: dc.relationDrug and Chemical Toxicology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectArrabidaea brachypoda-
Palavras-chave: dc.subjectatomic force microscope-
Palavras-chave: dc.subjectcell membrane-
Palavras-chave: dc.subjectchemotherapy-
Palavras-chave: dc.subjectnatural products-
Título: dc.titleUnusual dimeric flavonoids (brachydins) induce ultrastructural membrane alterations associated with antitumor activity in cancer cell lines-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.