LncRNAs of Saccharomyces cerevisiae bypass the cell cycle arrest imposed by ethanol stress

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorMax Planck Institute for Heart and Lung Research-
Autor(es): dc.creatorLázari, Lucas Cardoso-
Autor(es): dc.creatorWolf, Ivan Rodrigo-
Autor(es): dc.creatorSchnepper, Amanda Piveta-
Autor(es): dc.creatorValente, Guilherme Targino-
Data de aceite: dc.date.accessioned2025-08-21T17:43:15Z-
Data de disponibilização: dc.date.available2025-08-21T17:43:15Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1371/journal.pcbi.1010081-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/240146-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/240146-
Descrição: dc.descriptionEthanol alters many subsystems of Saccharomyces cerevisiae, including the cell cycle. Two ethanol-responsive lncRNAs in yeast interact with cell cycle proteins, and here, we investigated the role of these RNAs in cell cycle. Our network dynamic modeling showed that higher and lower ethanol-tolerant strains undergo cell cycle arrest in mitosis and G1 phases, respectively, during ethanol stress. The higher population rebound of the lower ethanol-tolerant phenotype after stress relief responds to the late phase arrest. We found that the lncRNA lnc9136 of SEY6210 (a lower ethanol-tolerant strain) induces cells to skip mitosis arrest. Simulating an overexpression of lnc9136 and analyzing CRISPR–Cas9 mutants lacking this lncRNA suggest that lnc9136 induces a regular cell cycle even under ethanol stress, indirectly regulating Swe1p and Clb1/2 by binding to Gin4p and Hsl1p. Notably, lnc10883 of BY4742 (a higher ethanol-tolerant strain) does not prevent G1 arrest in this strain under ethanol stress. However, lnc19883 circumvents DNA and spindle damage checkpoints, maintaining a functional cell cycle by interacting with Mec1p or Bub1p even in the presence of DNA/spindle damage. Overall, we present the first evidence of direct roles for lncRNAs in regulating yeast cell cycle proteins, the dynamics of this system in different ethanol-tolerant phenotypes, and a new yeast cell cycle model.-
Descrição: dc.descriptionDepartment of Parasitology Institute of Biomedical Sciences Sāo Paulo University (USP)-
Descrição: dc.descriptionDepartment of Bioprocess and Biotechnology School of Agriculture Sao Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Bioscience at Botucatu Sao Paulo State University (UNESP)-
Descrição: dc.descriptionMax Planck Institute for Heart and Lung Research-
Descrição: dc.descriptionDepartment of Bioprocess and Biotechnology School of Agriculture Sao Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Bioscience at Botucatu Sao Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationPLoS Computational Biology-
???dc.source???: dc.sourceScopus-
Título: dc.titleLncRNAs of Saccharomyces cerevisiae bypass the cell cycle arrest imposed by ethanol stress-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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