Regulation of interleukin-6 and matrix metalloproteinases syntheses by bioflavonoids and photobiomodulation in human gingival fibroblasts

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorRibeirão Preto University (UNAERP)-
Autor(es): dc.creatorCardoso, Laís Medeiros-
Autor(es): dc.creatorPansani, Taisa Nogueira-
Autor(es): dc.creatorCosta, Carlos Alberto de Souza-
Autor(es): dc.creatorBasso, Fernanda Gonçalves-
Data de aceite: dc.date.accessioned2025-08-21T23:01:55Z-
Data de disponibilização: dc.date.available2025-08-21T23:01:55Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s10103-022-03579-z-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/240129-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/240129-
Descrição: dc.descriptionThis study aimed to evaluate the separately effects of bioflavonoids proanthocyanidins, from grape seed extract (GSE) and synthetic naringenin (NA), as well as photobiomodulation (PBM) by low-level laser therapy on interleukin (IL)-6 and matrix metalloproteinases (MMPs) syntheses by human gingival fibroblasts (HGF). For this purpose, a connective tissue exposure (ulceration) model of HGF, stimulated with tumor necrosis factor-alpha (TNF-α), was used. Initially, the highest non-cytotoxic and non-genotoxic concentrations of bioflavonoids were determined by cell viability and micronuclei formation assays. Then, HGF were exposed to different stimuli: culture medium (negative control), dimethyl sulfoxide (DMSO), TNF-α, NA, GSE, TNF-α + NA, TNF-α + GSE, PBM (3 J/cm2, 0.025 W, 780 nm), and TNF-α + PBM. Next, IL-6, MMP-2, and MMP-9 syntheses were assessed. The concentration of 10 μg/mL of bioflavonoids increased cell viability at 24 and 48 h and did not present cytotoxic or genotoxic effects on HGF after 24, 48, and 72 h of contact. This concentration was selected for the assessment of bioflavonoids potential in modulating inflammatory mediators. TNF-α exposure enhanced IL-6 (170%), MMP-2 (10%), and MMP-9 (20%) syntheses, while a decrease of MMP-2 by 55% after exposure to TNF-α + GSE and 20% after TNF-α + NA and TNF-α + PBM was observed. MMP-9 synthesis was decreased by 35% after TNF-α + NA, 20% after TNF-α + GSE, and 30% after PBM. IL-6 was down-regulated by GSE in the presence of TNF-α (80%). In conclusion, TNF-α up-regulated IL-6 and MMPs, while bioflavonoids and PBM down-regulated MMP-2 and MMP-9 syntheses; GSE also decreased IL-6 synthesis, demonstrating the individual promising potential of these therapies for ulceration management.-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP, 1680 Humaitá Street, São Paulo-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP, 1680 Humaitá Street, São Paulo-
Descrição: dc.descriptionDepartment of Dentistry Ribeirão Preto University (UNAERP), 2201 Costábile Romano Avenue, São Paulo-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP, 1680 Humaitá Street, São Paulo-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP, 1680 Humaitá Street, São Paulo-
Idioma: dc.languageen-
Relação: dc.relationLasers in Medical Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectGingival fibroblasts-
Palavras-chave: dc.subjectLow-level laser therapy-
Palavras-chave: dc.subjectMatrix metalloproteinases-
Palavras-chave: dc.subjectNaringenin-
Palavras-chave: dc.subjectProanthocyanidins-
Título: dc.titleRegulation of interleukin-6 and matrix metalloproteinases syntheses by bioflavonoids and photobiomodulation in human gingival fibroblasts-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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