Effect of testing protocol and within-pen prevalence on the detection of Mycoplasma hyopneumoniae DNA in oral fluid samples

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Autor(es): dc.contributorIowa State University-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorIDEXX Laboratories Inc.-
Autor(es): dc.contributorPIC®-
Autor(es): dc.contributorNational Animal Disease Center-
Autor(es): dc.creatorSilva, Ana Paula S. Poeta-
Autor(es): dc.creatorStorino, Gabriel Y.-
Autor(es): dc.creatorFerreyra, Franco S. Matias-
Autor(es): dc.creatorZhang, Min-
Autor(es): dc.creatorMiller, Jessica M.-
Autor(es): dc.creatorHarmon, Karen M.-
Autor(es): dc.creatorGauger, Phillip C.-
Autor(es): dc.creatorWitbeck, Wendy-
Autor(es): dc.creatorDoolittle, Kent-
Autor(es): dc.creatorZimmerman, Silvia-
Autor(es): dc.creatorWang, Chong-
Autor(es): dc.creatorDerscheid, Rachel J.-
Autor(es): dc.creatorClavijo, Maria J.-
Autor(es): dc.creatorArruda, Bailey L.-
Autor(es): dc.creatorZimmerman, Jeffrey J.-
Data de aceite: dc.date.accessioned2025-08-21T21:30:05Z-
Data de disponibilização: dc.date.available2025-08-21T21:30:05Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.prevetmed.2022.105670-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/240064-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/240064-
Descrição: dc.descriptionCombinations of 2 nucleic acid extractions and 3 Mycoplasma hyopneumoniae (MHP) PCRs (namely Protocol 1, 2, 3, and 4) were compared in terms of the probability of detecting DNA in pen-based oral fluid samples as a function of within-pen MHP prevalence. Oral fluid samples were created by randomly assigning 39 7-week old pigs to one of 5 pens, i.e., negative control pen (3 pigs) and 4 pens of 9 pigs each that differed in the proportion of MHP-inoculated pigs (1, 3, 6, or 9). Deep tracheal swabs were collected twice weekly to establish individual pig MHP infection status and derive within-pen prevalence estimation. On DPI 3, tracheal swabs from 15 of 19 inoculated pigs were MHP DNA positive. Oral fluids (n = 320) were collected daily from − 4 to 59 days post inoculation (DPI). Using a piecewise exponential model to account for within-pen transmission dynamics followed by a mixed-effect logistic regression, the probability of detecting MHP DNA in oral fluids was positively associated with within-pen prevalence (P < 0.0001) and differed among test protocols. MHP DNA was detected in 173 oral fluid samples with Protocol 3 versus 148, 134, and 101 with Protocols 4, 2, and 1, respectively. At 100% within-pen prevalence, the probability of detecting MHP DNA in oral fluids was highest using Protocol 3 (95.7%), followed by Protocols 4 (70.1%), 2 (60.1%), and 1 (34.0%). The fact that PCR protocols performed differently suggests that further improvements in extraction methods and MHP PCRs are possible. In the field, the dynamics of MHP infections should be taken into account if using oral fluid samples in surveillance.-
Descrição: dc.descriptionIowa Pork Producers Association-
Descrição: dc.descriptionDepartment of Veterinary Diagnostic and Production Animal Medicine Iowa State University-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, SP-
Descrição: dc.descriptionDepartment of Statistics Iowa State University-
Descrição: dc.descriptionIDEXX Laboratories Inc.-
Descrição: dc.descriptionPig Improvement Company PIC®-
Descrição: dc.descriptionUS Department of Agriculture Agricultural Research Service National Animal Disease Center-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, SP-
Descrição: dc.descriptionIowa Pork Producers Association: 18-133 IPPA-
Idioma: dc.languageen-
Relação: dc.relationPreventive Veterinary Medicine-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCutoff-
Palavras-chave: dc.subjectDNA-
Palavras-chave: dc.subjectMycoplasma hyopneumoniae-
Palavras-chave: dc.subjectOral fluid-
Palavras-chave: dc.subjectPCR-
Palavras-chave: dc.subjectProbability of detection-
Palavras-chave: dc.subjectTracheal swab-
Título: dc.titleEffect of testing protocol and within-pen prevalence on the detection of Mycoplasma hyopneumoniae DNA in oral fluid samples-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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