Improving the cost effectiveness of enhanced green fluorescent protein production using recombinant Escherichia coli BL21 (DE3): Decreasing the expression inducer concentration

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatordos Santos, Nathalia Vieira-
Autor(es): dc.creatorPereira, Jorge Fernando Brandão-
Autor(es): dc.creatorPedrolli, Danielle Biscaro-
Autor(es): dc.creatorSantos-Ebinuma, Valéria De Carvalho-
Autor(es): dc.creatorValentini, Sandro Roberto-
Autor(es): dc.creatorLopes, Camila-
Autor(es): dc.creatorDupont, Jana-
Data de aceite: dc.date.accessioned2025-08-21T23:15:35Z-
Data de disponibilização: dc.date.available2025-08-21T23:15:35Z-
Data de envio: dc.date.issued2022-12-22-
Data de envio: dc.date.issued2022-12-22-
Data de envio: dc.date.issued2019-04-08-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/238474-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/238474-
Descrição: dc.descriptionGreen Fluorescent Protein (GFP) is a globular protein used as biosensor and biomarker in medical and industrial fields. However, due to the expensive production costs of expressing proteins using high-cost inducers like isopropyl-β-D-1-thiogalactopyranoside (IPTG), the number of GFP applications are still scarce. This work studied the production of Enhanced GFP (EGFP) using Escherichia coli BL21 (DE3) [pLysS; pET28(a)], aiming to increase its yield and reduce costs. Firstly, the influence of agitation rate, induction time and concentration of IPTG in the production of EGFP were evaluated, but only the first two parameters were significant. Subsequently, aiming to reduce costs related to the use of inducer, the IPTG concentration (0.005, 0.010 and 0.025 mM) was decreased and, interestingly, the production levels were maintained or increased. These results show that a proper choice of production conditions, particularly through the decrease of inducer concentration, is effective to reduce the upstream production costs and guarantee high EGFP expression.-
Descrição: dc.descriptionOutra-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionFundação para a Ciência e a Tecnologia (FCT)-
Descrição: dc.descriptionDepartment of Bioprocesses and Biotechnology, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, Brazil.-
Descrição: dc.descriptionFaculty of Bioscience Engineering, Gent University, Gent, Belgium.-
Descrição: dc.descriptionDepartment of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, Brazil.-
Descrição: dc.descriptionFAPESP/FCT 2014/19793-3-
Descrição: dc.descriptionCAPES 001-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Publicador: dc.publisherWiley-
Relação: dc.relationhttps://iubmb.onlinelibrary.wiley.com/doi/abs/10.1002/bab.1749-
Relação: dc.relationBiotechnology and Applied Biochemistry-
Direitos: dc.rightsinfo:eu-repo/semantics/openAccess-
Palavras-chave: dc.subjectbiomarker-
Palavras-chave: dc.subjectproduction-
Palavras-chave: dc.subjectEscherichia coli-
Palavras-chave: dc.subjectgreen fluorescent protein-
Palavras-chave: dc.subjectprotein expression inducer-
Palavras-chave: dc.subjectIPTG-
Título: dc.titleImproving the cost effectiveness of enhanced green fluorescent protein production using recombinant Escherichia coli BL21 (DE3): Decreasing the expression inducer concentration-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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