Colletotrichum abscissum: Detection on symptomless citrus leaves and symptomatic citrus flowers using high-resolution melting analysis

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorGasparoto, Maria Cândida de Godoy-
Autor(es): dc.creatorGama, Andre Bueno-
Autor(es): dc.creatorWang, Nan-Yi-
Autor(es): dc.creatorRebello, Carolina Suguinoshita-
Autor(es): dc.creatorDewdney, Megan M.-
Autor(es): dc.creatorPeres, Natalia A.-
Data de aceite: dc.date.accessioned2025-08-21T18:10:33Z-
Data de disponibilização: dc.date.available2025-08-21T18:10:33Z-
Data de envio: dc.date.issued2022-12-21-
Data de envio: dc.date.issued2022-12-21-
Data de envio: dc.date.issued2021-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/238461-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/238461-
Descrição: dc.descriptionHigh-resolution melting (HRM) analysis has been recently applied for the diagnosis of plant diseases. This cost-effective method is advantageous over standard PCR as it does not require DNA band visualization or the use of probes necessary for quantitative PCR. Colletotrichum abscissum causes postbloom fruit drop of citrus (PFD) and survives asymptomatically on vegetative tissue. Quiescent appressoria on citrus leaves are the primary source of inoculum between flowering periods. Early PFD symptoms may be misidentified as physical damage on citrus flowers. Our objectives were to validate an HRM-based method to rapidly identify C. abscissum on citrus leaves and flowers. We screened seven previously published primer pairs and concluded that the most effective set of primers was CaITS-F and CaITS-R815. We evaluated three different DNA extraction methods, two with a purification step (DNeasy Plant Mini and PowerSoil kits) and a faster method without a purification step (buffer GEB2). We inoculated citrus leaves with C. abscissum conidial suspensions of varying concentrations and HRM detected as few as 200 C. abscissum spores using DNeasy Plant Mini or DNeasy PowerSoil kits for DNA extraction. As expected, samples extracted with GEB2 buffer reduced HRM sensitivity. We further collected field samples from areas with high and low C. abscissum inoculum levels. The HRM method could detect the pathogen in high inoculum areas from both leaves and flowers regardless of the extraction method used. In summary, we describe a new molecular diagnostic tool to detect C. abscissum on citrus during its asymptomatic and symptomatic stages.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionPreprint-
Descrição: dc.descriptionUnesp - Campus de Registro-
Descrição: dc.descriptionUniversity of Florida-
Descrição: dc.description2019/02604-7-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Publicador: dc.publisherWiley-
Relação: dc.relationPlant Pathology-
Direitos: dc.rightsinfo:eu-repo/semantics/openAccess-
Palavras-chave: dc.subjectColletotrichum acutatum-
Palavras-chave: dc.subjectAppressoria-
Palavras-chave: dc.subjectAsymptomatic detection-
Palavras-chave: dc.subjectColletotrichum gloeosporioides-
Palavras-chave: dc.subjectInoculum detection-
Título: dc.titleColletotrichum abscissum: Detection on symptomless citrus leaves and symptomatic citrus flowers using high-resolution melting analysis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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