Cytotoxic Cyclotides from Anchietea pyrifolia, a South American Plant Species

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniv Frontier UFRO-
Autor(es): dc.contributorUniversidade Federal de Santa Catarina (UFSC)-
Autor(es): dc.creatorFernandez-Bobey, Antonio-
Autor(es): dc.creatorPinto, Meri Emili Ferreira-
Autor(es): dc.creatorAlmeida, Larissa Costa de-
Autor(es): dc.creatorSouza, Bibiana Monson de-
Autor(es): dc.creatorDias, Nathalia Baptista-
Autor(es): dc.creatorDe Paula-Souza, Juliana-
Autor(es): dc.creatorCilli, Eduardo Maffud-
Autor(es): dc.creatorLopes, Norberto Peporine-
Autor(es): dc.creatorCosta-Lotufo, Leticia Veras-
Autor(es): dc.creatorPalma, Mario Sergio-
Autor(es): dc.creatorBolzani, Vanderlan da Silva-
Data de aceite: dc.date.accessioned2025-08-21T16:56:03Z-
Data de disponibilização: dc.date.available2025-08-21T16:56:03Z-
Data de envio: dc.date.issued2022-11-29-
Data de envio: dc.date.issued2022-11-29-
Data de envio: dc.date.issued2022-08-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1021/acs.jnatprod.1c01129-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/237850-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/237850-
Descrição: dc.descriptionCyclotides are mini-proteins with potent bioactivities and outstanding potential for agricultural and pharmaceutical applications. More than 450 different plant cyclotides have been isolated from six angiosperm families. In Brazil, studies involving this class of natural products are still scarce, despite its rich floristic diversity. Herein were investigated the cyclotides from Anchietea pyrifolia roots, a South American medicinal plant from the family Violaceae. Fourteen putative cyclotides were annotated by LC-MS. Among these, three new bracelet cyclotides, anpy A-C, and the known cycloviolacins O4 (cyO4) and O17 (cyO17) were sequenced through a combination of chemical and enzymatic reactions followed by MALDI-MS/MS analysis. Their cytotoxic activity was evaluated by a cytotoxicity assay against three human cancer cell lines (colorectal carcinoma cells: HCT 116 and HCT 116 TP53(-/-) and breast adenocarcinoma, MCF 7). For all assays, the IC50 values of isolated compounds ranged between 0.8 and 7.3 mu M. CyO17 was the most potent cyclotide for the colorectal cancer cell lines (IC50, 0.8 and 1.2 mu M). Furthermore, the hemolytic activity of anpy A and B, cyO4, and cyO17 was assessed, and the cycloviolacins were the least hemolytic (HD50 > 156 mu M). This work sheds light on the cytotoxic effects of the anpy cyclotides against cancer cells. Moreover, this study expands the number of cyclotides obtained to date from Brazilian plant biodiversity and adds one more genus containing these molecules to the list of the Violaceae family.-
Descrição: dc.descriptionINCT Program-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionThematic Projects-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionJacqueline Nakau Mendonca-
Descrição: dc.descriptionSao Paulo State Univ, UNESP, Inst Chem, Nucleus Bioassays Biosynth & Ecophysiol Nat Prod, BR-14800060 Sao Paulo, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, UNESP, Inst Biosci, Dept Basic & Appl Biol,Lab Struct Biol & Zoochem, BR-13506900 Sao Paulo, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Inst Biomed Sci, Dept Pharmacol, BR-05508900 Sao Paulo, Brazil-
Descrição: dc.descriptionUniv Frontier UFRO, Sci & Technol Bioresource Nucleus BIOREN, Temuco 4881176, Chile-
Descrição: dc.descriptionFed Univ Santa Catarina UFSC, Dept Bot, BR-88040535 Florianopolis, SC, Brazil-
Descrição: dc.descriptionUniv Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Nucleus Res Nat & Synthet Prod NPPNS, BR-14040903 Sao Paulo, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, UNESP, Inst Chem, Nucleus Bioassays Biosynth & Ecophysiol Nat Prod, BR-14800060 Sao Paulo, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, UNESP, Inst Biosci, Dept Basic & Appl Biol,Lab Struct Biol & Zoochem, BR-13506900 Sao Paulo, Brazil-
Descrição: dc.descriptionINCT Program: 465637/2014-0-
Descrição: dc.descriptionINCT Program: 2014/50926-0-
Descrição: dc.descriptionFAPESP: 2013/07600-3-
Descrição: dc.descriptionFAPESP: 2014/25222-9-
Descrição: dc.descriptionFAPESP: 142286/2016-
Descrição: dc.descriptionFAPESP: 2019/26550-3-
Descrição: dc.descriptionThematic Projects: 2016/16212-5-
Descrição: dc.descriptionThematic Projects: 2020/02207-5-
Descrição: dc.description: 2017/17098-4-
Descrição: dc.description: 2019/04381-5-
Formato: dc.format8-
Idioma: dc.languageen-
Publicador: dc.publisherAmer Chemical Soc-
Relação: dc.relationJournal Of Natural Products-
???dc.source???: dc.sourceWeb of Science-
Título: dc.titleCytotoxic Cyclotides from Anchietea pyrifolia, a South American Plant Species-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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