Cytogenetic Mapping of Cattle BAC Probes for the Hypothetical Ancestral Karyotype of the Family Cervidae

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorCent European Inst Technol-
Autor(es): dc.creatorBernegossi, Agda Maria-
Autor(es): dc.creatorVozdova, Miluse-
Autor(es): dc.creatorCernohorska, Halina-
Autor(es): dc.creatorKubickova, Svatava-
Autor(es): dc.creatorGalindo, David Javier-
Autor(es): dc.creatorKadlcikova, Dita-
Autor(es): dc.creatorRubes, Jiri-
Autor(es): dc.creatorDuarte, Jose Mauricio Barbanti-
Data de aceite: dc.date.accessioned2025-08-21T22:05:21Z-
Data de disponibilização: dc.date.available2025-08-21T22:05:21Z-
Data de envio: dc.date.issued2022-11-29-
Data de envio: dc.date.issued2022-11-29-
Data de envio: dc.date.issued2022-08-18-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1159/000525592-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/237673-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/237673-
Descrição: dc.descriptionCervids are characterized by their greatest karyotypic diversity among mammals. A great diversity of chromosome numbers in notably similar morphological groups leads to the existence of several complexes of cryptic species and taxonomic uncertainties. Some deer lineages, such as those of Neotropical deer, stand out for a rapid chromosomal reorganization and intraspecific chromosome polymorphisms, which have not been properly explored yet. For that reason, we contribute to the study of deer karyotype diversity and taxonomy by producing and characterizing new molecular cytogenetic markers for the gray brocket deer (Subulo gouazoubira), a deer species that retained the hypothetical ancestral karyotype of Cervidae. We used bacterial artificial chromosome (BAC) clones derived from the cattle genome (Bos taurus) as markers, which were hybridized on S. gouazoubira metaphase chromosomes. In total, we mapped 108 markers, encompassing all gray brocket deer chromosomes, except the Y chromosome. The detailed analysis of fluorescent in situ hybridization results showed 6 fissions and 1 fusion as interchromosomal rearrangements that have separated cattle and gray brocket deer karyotypes. Each group of BAC probes derived from bovine chromosome pairs 1, 2, 5, 6, 8, and 9 showed hybridization signals on 2 different chromosomes, while pairs 28 and 26 are fused in tandem in a single acrocentric chromosome in S. gouazoubira. Furthermore, the BAC markers detected the occurrence of intrachromosomal rearrangements in the S. gouazoubira chromosomes homologous to pair 1 and the X chromosome of cattle. We present a karyotypic map of the 108 new markers, which will be of great importance for future karyotypic evolution studies in cervids and, consequently, help in their conservation and taxonomy resolution.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCzech Science Foundation (GACR)-
Descrição: dc.descriptionMinistry of Agriculture of the Czech Republic-
Descrição: dc.descriptionNational Fund for Scientific, Technological Development and Technological Innovation (FONDECYT)-
Descrição: dc.descriptionNational Council for Science, Technology and Technological Innovation (CONCYTEC) Peru-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionSao Paulo State Univ UNESP, Sch Agr & Vet Sci, Deer Res & Conservat Ctr NUPECCE, Jaboticabal, Brazil-
Descrição: dc.descriptionCent European Inst Technol, Vet Res Inst, Brno, Czech Republic-
Descrição: dc.descriptionSao Paulo State Univ UNESP, Sch Agr & Vet Sci, Deer Res & Conservat Ctr NUPECCE, Jaboticabal, Brazil-
Descrição: dc.descriptionFAPESP: 2017/07014-8-
Descrição: dc.descriptionFAPESP: 2018/25769-9-
Descrição: dc.descriptionFAPESP: 2019/20810-3-
Descrição: dc.descriptionFAPESP: 2019/06940-1-
Descrição: dc.descriptionCzech Science Foundation (GACR): 20-22517J-
Descrição: dc.descriptionMinistry of Agriculture of the Czech Republic: RO 0520-
Descrição: dc.descriptionNational Council for Science, Technology and Technological Innovation (CONCYTEC) Peru: 116-2017-FONDECYT-
Formato: dc.format8-
Idioma: dc.languageen-
Publicador: dc.publisherKarger-
Relação: dc.relationCytogenetic And Genome Research-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectChromosomal evolution-
Palavras-chave: dc.subjectFISH-
Palavras-chave: dc.subjectGray brocket deer-
Palavras-chave: dc.subjectMolecular cytogenetics-
Título: dc.titleCytogenetic Mapping of Cattle BAC Probes for the Hypothetical Ancestral Karyotype of the Family Cervidae-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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