Effects of α and β-adrenergic signaling on innate immunity and Porphyromonas gingivalis virulence in an invertebrate model

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorMoraes, Renata Mendonça-
Autor(es): dc.creatorGarcia, Maíra Terra-
Autor(es): dc.creatorStossi, Fabio-
Autor(es): dc.creatorJunqueira, Juliana Campos-
Autor(es): dc.creatorAnbinder, Ana Lia-
Autor(es): dc.creatorBarros, Patrícia Pimentel de-
Data de aceite: dc.date.accessioned2025-08-21T17:12:58Z-
Data de disponibilização: dc.date.available2025-08-21T17:12:58Z-
Data de envio: dc.date.issued2022-10-16-
Data de envio: dc.date.issued2022-10-16-
Data de envio: dc.date.issued2022-09-19-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/237077-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/237077-
Descrição: dc.descriptionTo investigate the role of adrenergic signaling (AS) in the host immune response and Porphyromonas gingivalis virulence, we compared norepinephrine (NE) and isoproterenol (ISO) responses in Galleria mellonella. P. gingivalis infection was evaluated by survival; humoral immune responses (i.e., melanization and cecropin and gloverin mRNA expression); cellular immune responses (i.e., hemocyte count, nodulation by histology); and P. gingivalis recovery (CFU/mL). P. gingivalis was cultivated in the presence of ISO (PgISO) or NE and injected into the larvae for survival evaluation. Finally, we co-injected ISO and PgISO to evaluate the concomitant effects on the immune response and bacterial virulence. None of the ligands were toxic to the larvae; ISO increased hemocyte number, even after P. gingivalis infection, by mobilizing sessile hemocytes in a β-adrenergic-specific manner, while NE showed the opposite effect. ISO treatment reduced larval mortality and the number of recovered bacteria, while NE increased mortality and showed no effect on bacterial recovery. ISO and NE had similar effects on melanization and decreased the expression of cecropin. Although co-cultivation with NE and ISO increased the gene expression of bacterial virulence factors in vitro, only the injection of PgISO increased larval death, which was partially reversed by circulating ISO. Therefore, α- and β-adrenergic signaling had opposite effects after P. gingivalis infection. Ultimately, the catecholamine influence on the immune response overcame the effect of more virulent strains. The effect of AS directly on the pathogen found in vitro did not translate to the in vivo setting.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionPreprint-
Descrição: dc.descriptionSão Paulo State University (Unesp), Institute of Science and Technology, Bioscience and Diagnosis Department, São José dos Campos, Brazil.-
Descrição: dc.descriptionSão Paulo State University (Unesp), Institute of Science and Technology, Bioscience and Diagnosis Department, São José dos Campos, Brazil-
Descrição: dc.descriptionDepartment of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas and GCC Center for Advanced Microscopy and Image Informatics, Houston, Texas.-
Descrição: dc.descriptionSão Paulo State University (Unesp), Institute of Science and Technology, Bioscience and Diagnosis Department, São José dos Campos, Brazil e Multicampi School of Medical Sciences, Federal University of Rio Grande do Norte (UFRN), Caicó, RN, Brazil-
Descrição: dc.descriptionSão Paulo State University (Unesp), Institute of Science and Technology, Bioscience and Diagnosis Department, São José dos Campos, Brazil.-
Descrição: dc.descriptionSão Paulo State University (Unesp), Institute of Science and Technology, Bioscience and Diagnosis Department, São José dos Campos, Brazil.-
Descrição: dc.descriptionFAPESP 2017/26461-5-
Descrição: dc.descriptionFAPESP 2018/ 21701-0-
Descrição: dc.descriptionFAPESP 2018/25933-3-
Descrição: dc.descriptionNIH (DK56338, CA125123)-
Descrição: dc.descriptionCPRIT (RP170719)-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Publicador: dc.publisherTaylo&Francis-
Relação: dc.relationVirulence-
Direitos: dc.rightsinfo:eu-repo/semantics/openAccess-
Palavras-chave: dc.subjectGalleria mellonella-
Palavras-chave: dc.subjectPorphyromonas gingivalis-
Palavras-chave: dc.subjectAdrenergic signaling-
Palavras-chave: dc.subjectInvertebrates-
Palavras-chave: dc.subjectInnate immunity-
Título: dc.titleEffects of α and β-adrenergic signaling on innate immunity and Porphyromonas gingivalis virulence in an invertebrate model-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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