Establishment and multiapproach characterization of Amblyomma sculptum (Acari: Ixodidae) cell line (ASE-14) from Brazil

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorPontifical Catholic University of Paraná (PUCPR)-
Autor(es): dc.creatorLima-Duarte, Leidiane-
Autor(es): dc.creatorCastro-Santiago, Ana Carolina-
Autor(es): dc.creatorCamargo, Jaqueline Valéria-
Autor(es): dc.creatorFerretti, Ana Beatriz Stein Machado-
Autor(es): dc.creatorAnholeto, Luís Adriano-
Autor(es): dc.creatorPereira, Melissa Carolina-
Autor(es): dc.creatorIkeda, Priscila-
Autor(es): dc.creatorPerez, Carlos Alberto-
Autor(es): dc.creatorSanches, Gustavo Seron-
Autor(es): dc.creatorCamargo-Mathias, Maria Izabel-
Autor(es): dc.creatorCabral-de-Mello, Diogo Cavalcanti-
Autor(es): dc.creatorMachado, Rosangela Zacarias-
Autor(es): dc.creatorAndré, Marcos Rogério-
Autor(es): dc.creatorBarros-Battesti, Darci Moraes-
Data de aceite: dc.date.accessioned2025-08-21T15:36:46Z-
Data de disponibilização: dc.date.available2025-08-21T15:36:46Z-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2022-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ttbdis.2022.101951-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/234370-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/234370-
Descrição: dc.descriptionThe establishment and characterization of the ASE-14 cell line derived from embryos of Amblyomma sculptum is described here. Primary cultures were started, and after 60 days of culturing a confluent monolayer was formed and the first subculture was then carried out. After this, new subcultures were carried out every 4 weeks. Cryopreservation of cells was successful only after the 14th subculture. We compared the chromosomes of the ASE-14 cell line with those of parental ticks. Cytogenetic analysis revealed occurrences of variable and increased diploid numbers in the ASE-14 cell line in comparison with adult ticks, probably through polyploidization events, chromosome fusions and translocations, which allowed generation of cells with distinct diploid numbers. Confirmation of the origin of the A. sculptum cell line was obtained through conventional PCR and sequencing of a fragment of the mitochondrial 16S rRNA gene. In addition, no DNA from Anaplasma marginale, Anaplasma spp., Babesia/Theileria spp., Bartonella spp., Coxiella spp., Ehrlichia canis, Mycoplasma spp. or Rickettsia spp. was detected in the cells through PCR assays. Cytological analyses were performed using live phase contrast microscopy and cytocentrifuge smears stained with Giemsa, while periodic acid-Schiff and bromophenol blue staining techniques were used to detect polysaccharides and protein, respectively. In conclusion, a new cell line derived from embryos of A. sculptum was generated and characterized in this study. The ASE-14 cell line was deposited in the Tick Cell Biobank at the University of Liverpool, and in the Tick Cell Biobank South America Outpost at the Oswaldo Cruz Foundation (FIOCRUZ). The ASE-14 cell line is an important addition to the existing panel of tick cell lines and can be used as a tool for advancing research in various areas of the virology, bacteriology, biology and control of this tick.-
Descrição: dc.descriptionDepartment of Preventive Veterinary Medicine and Animal Science School of Veterinary Medicine University of São Paulo, SP-
Descrição: dc.descriptionDepartment of Pathology Theriogenology and One Health São Paulo State University “Julio de Mesquita Filho” (UNESP), SP-
Descrição: dc.descriptionDepartment of General and Applied Biology Institute of Biosciences São Paulo State University “Julio de Mesquita Filho” (UNESP), SP-
Descrição: dc.descriptionLuiz de Queiroz College of Agriculture (ESALQ) University of São Paulo, SP-
Descrição: dc.descriptionPostgraduate Program on Animal Science Pontifical Catholic University of Paraná (PUCPR), PR-
Descrição: dc.descriptionDepartment of Pathology Theriogenology and One Health São Paulo State University “Julio de Mesquita Filho” (UNESP), SP-
Descrição: dc.descriptionDepartment of General and Applied Biology Institute of Biosciences São Paulo State University “Julio de Mesquita Filho” (UNESP), SP-
Idioma: dc.languageen-
Relação: dc.relationTicks and Tick-borne Diseases-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBrazil-
Palavras-chave: dc.subjectchromosome-
Palavras-chave: dc.subjecttick cell line-
Título: dc.titleEstablishment and multiapproach characterization of Amblyomma sculptum (Acari: Ixodidae) cell line (ASE-14) from Brazil-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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