Immunoassay platform with surface-enhanced resonance Raman scattering for detecting trace levels of SARS-CoV-2 spike protein

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorBistaffa, Maria J.-
Autor(es): dc.creatorCamacho, Sabrina A.-
Autor(es): dc.creatorPazin, Wallance M.-
Autor(es): dc.creatorConstantino, Carlos J.L.-
Autor(es): dc.creatorOliveira, Osvaldo N.-
Autor(es): dc.creatorAoki, Pedro H.B.-
Data de aceite: dc.date.accessioned2025-08-21T16:33:04Z-
Data de disponibilização: dc.date.available2025-08-21T16:33:04Z-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2022-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.talanta.2022.123381-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/234315-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/234315-
Descrição: dc.descriptionThe early diagnosis of Coronavirus disease (COVID-19) requires either an accurate detection of genetic material or a sensitive detection of viral proteins. In this work, we designed an immunoassay platform for detecting trace levels of SARS-CoV-2 spike (S) protein. It is based on surface-enhanced resonance Raman scattering (SERRS) of methylene blue (MB) adsorbed onto spherical gold nanoparticles (AuNPs) and coated with a 6 nm silica shell. The latter shell in the SERRS nanoprobe prevented aggregation and permitted functionalization with SARS-CoV-2 antibodies. Specificity of the immunoassay was achieved by combining this functionalization with antibody immobilization on the cover slides that served as the platform support. Different concentrations of SARS-CoV-2 antigen could be distinguished and the lack of influence of interferents was confirmed by treating SERRS data with the multidimensional projection technique Sammon's mapping. With SERRS using a laser line at 633 nm, the lowest concentration of spike protein detected was 10 pg/mL, achieving a limit of detection (LOD) of 0.046 ng/mL (0.60 pM). This value is comparable to the lowest concentrations in the plasma of COVID-19 patients at the onset of symptoms, thus indicating that the SERRS immunoassay platform may be employed for early diagnosis.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionInstituto Nacional de Ciência e Tecnologia em Eletrônica Orgânica-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Sciences Humanities and Languages, Assis, SP-
Descrição: dc.descriptionIFSC São Carlos Institute of Physics University of São Paulo (USP), SP-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Technology and Applied Sciences, SP-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Sciences Humanities and Languages, Assis, SP-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Technology and Applied Sciences, SP-
Descrição: dc.descriptionFAPESP: 2018/14692–5-
Descrição: dc.descriptionFAPESP: 2018/16713–0-
Descrição: dc.descriptionFAPESP: 2018/22214–6-
Descrição: dc.descriptionFAPESP: 2020/12129–1-
Descrição: dc.descriptionCNPq: 403713/2016–1-
Descrição: dc.descriptionFAPESP: EMU 2017/03879–4-
Descrição: dc.descriptionFAPESP: FAPESP-
Idioma: dc.languageen-
Relação: dc.relationTalanta-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectConjugation in gold nanoparticles-
Palavras-chave: dc.subjectResonant Raman molecules-
Palavras-chave: dc.subjectSARS-CoV-2 antibodies-
Palavras-chave: dc.subjectSARS-CoV-2 spike protein detection-
Palavras-chave: dc.subjectSERRS immunoassay Platform-
Palavras-chave: dc.subjectSilica shell functionalization-
Título: dc.titleImmunoassay platform with surface-enhanced resonance Raman scattering for detecting trace levels of SARS-CoV-2 spike protein-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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