The Long Non-Coding RNA SNHG12 as a Mediator of Carboplatin Resistance in Ovarian Cancer via Epigenetic Mechanisms

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Autor(es): dc.contributorLillebaelt University Hospital of Southern Denmark-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorCIPE—C. Camargo Cancer Center-
Autor(es): dc.contributorUniversity of Southern Denmark-
Autor(es): dc.creatorAbildgaard, Cecilie-
Autor(es): dc.creatorDo Canto, Luisa Matos-
Autor(es): dc.creatorRainho, Cláudia Aparecida-
Autor(es): dc.creatorMarchi, Fabio Albuquerque-
Autor(es): dc.creatorCalanca, Naiade-
Autor(es): dc.creatorWaldstrøm, Marianne-
Autor(es): dc.creatorSteffensen, Karina Dahl-
Autor(es): dc.creatorRogatto, Silvia Regina-
Data de aceite: dc.date.accessioned2025-08-21T17:41:23Z-
Data de disponibilização: dc.date.available2025-08-21T17:41:23Z-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2022-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/cancers14071664-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/234311-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/234311-
Descrição: dc.descriptionGenetic and epigenetic changes contribute to intratumor heterogeneity and chemotherapy resistance in several tumor types. LncRNAs have been implicated, directly or indirectly, in the epigenetic regulation of gene expression. We investigated lncRNAs that potentially mediate carboplatin-resistance of cell subpopulations, influencing the progression of ovarian cancer (OC). Four carboplatin-sensitive OC cell lines (IGROV1, OVCAR3, OVCAR4, and OVCAR5), their derivative resistant cells, and two inherently carboplatin-resistant cell lines (OVCAR8 and Ovc316) were subjected to RNA sequencing and global DNA methylation analysis. Integrative and cross-validation analyses were performed using external (The Cancer Genome Atlas, TCGA dataset, n = 111 OC samples) and internal datasets (n = 39 OC samples) to identify lncRNA candidates. A total of 4255 differentially expressed genes (DEGs) and 14529 differentially methylated CpG positions (DMPs) were identified comparing sensitive and resistant OC cell lines. The comparison of DEGs between OC cell lines and TCGA-OC dataset revealed 570 genes, including 50 lncRNAs, associated with carboplatin resistance. Eleven lncRNAs showed DMPs, including the SNHG12. Knockdown of SNHG12 in Ovc316 and OVCAR8 cells increased their sensitivity to carboplatin. The results suggest that the lncRNA SNHG12 contributes to carboplatin resistance in OC and is a potential therapeutic target. We demonstrated that SNHG12 is functionally related to epigenetic mechanisms.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartment of Clinical Genetics Lillebaelt University Hospital of Southern Denmark-
Descrição: dc.descriptionDepartment of Oncology Lillebaelt University Hospital of Southern Denmark-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionInternational Research Center CIPE—C. Camargo Cancer Center-
Descrição: dc.descriptionDepartment of Pathology Lillebaelt University Hospital of Southern Denmark-
Descrição: dc.descriptionInstitute of Regional Health Research Faculty of Health Sciences University of Southern Denmark-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionCAPES: 88887.310463/2018-00-
Idioma: dc.languageen-
Relação: dc.relationCancers-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectchemotherapy-
Palavras-chave: dc.subjectDNA methylation-
Palavras-chave: dc.subjectdrug resistance-
Palavras-chave: dc.subjectlncRNA-
Palavras-chave: dc.subjectovarian cancer-
Palavras-chave: dc.subjecttranscrip-tomic analysis-
Título: dc.titleThe Long Non-Coding RNA SNHG12 as a Mediator of Carboplatin Resistance in Ovarian Cancer via Epigenetic Mechanisms-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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