Natural latex serum: characterization and biocompatibility assessment using Galleria mellonella as an alternative in vivo model

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFederal University of Rio Grande do Norte (UFRN)-
Autor(es): dc.contributorUniversity of Aveiro-
Autor(es): dc.contributorTerasaki Institute for Biomedical Innovation (TIBI)-
Autor(es): dc.contributorFederal University of Triangulo Mineiro (UFTM)-
Autor(es): dc.creatorPegorin Brasil, Giovana Sant’Ana-
Autor(es): dc.creatorde Barros, Patrícia Pimentel-
Autor(es): dc.creatorMiranda, Matheus Carlos Romeiro-
Autor(es): dc.creatorde Barros, Natan Roberto-
Autor(es): dc.creatorJunqueira, Juliana Campos-
Autor(es): dc.creatorGomez, Alejandro-
Autor(es): dc.creatorHerculano, Rondinelli Donizetti-
Autor(es): dc.creatorde Mendonça, Ricardo José-
Data de aceite: dc.date.accessioned2025-08-21T22:15:37Z-
Data de disponibilização: dc.date.available2025-08-21T22:15:37Z-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1080/09205063.2021.2014027-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/233982-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/233982-
Descrição: dc.descriptionNatural latex serum (NLS) is one of the natural rubber latex fractions from Hevea brasiliensis tree, which is formed by centrifuged serum and is composed of proteins, acids, nucleotides, salts and carbohydrates. The proteins present in NLS have demonstrated several interesting biological properties, including angiogenic, healing, osteogenic, anti-inflammatory, antimicrobial, in addition to inducing neovascularization, bone formation and osseointegration. Thus, we proposed to characterize NLS by physicochemical techniques and to investigate the biocompatibility by toxicological assays and safety test in Galleria mellonella. Infrared spectrum showed vibrational bands characteristic of amide I, II and III that are linked to the protein content, which was confirmed by the High Performance Liquid Chromatography profile and by the Electrophoresis analysis. This material did not exhibit hemolytic (rate <0.5%) and cytotoxic effects (viability >70%) and was able to enhance the proliferation of fibroblasts (>600%) after 3 days. The pronounced proliferative effect observed in fibroblast cells can be explained by the presence of the fibroblast growth factor (FGF) like protein revealed by the Western blot test. Moreover, NLS did not provoke toxic effects (survival ∼ 80%) on the G. mellonella model, indicating that it is a biocompatible and safe material.-
Descrição: dc.descriptionInstitute of Chemistry São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Biotechnology and Bioprocess Engineering School of Pharmaceutical Sciences São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionMulticampi School of Medical Sciences Federal University of Rio Grande do Norte (UFRN), Rio Grande do Norte-
Descrição: dc.descriptionCentre for Environmental and Marine Studies (CESAM) University of Aveiro-
Descrição: dc.descriptionTerasaki Institute for Biomedical Innovation (TIBI)-
Descrição: dc.descriptionDepartment of Biochemistry Pharmacology and Physiology Federal University of Triangulo Mineiro (UFTM), Minas Gerais-
Descrição: dc.descriptionInstitute of Chemistry São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Biotechnology and Bioprocess Engineering School of Pharmaceutical Sciences São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP), São Paulo-
Formato: dc.format705-726-
Idioma: dc.languageen-
Relação: dc.relationJournal of Biomaterials Science, Polymer Edition-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectGalleria mellonella-
Palavras-chave: dc.subjectHevea brasiliensis-
Palavras-chave: dc.subjectHPLC-
Palavras-chave: dc.subjectNatural latex serum-
Palavras-chave: dc.subjecttoxicity-
Palavras-chave: dc.subjectWestern blot-
Título: dc.titleNatural latex serum: characterization and biocompatibility assessment using Galleria mellonella as an alternative in vivo model-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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