Diagnosis, risk factors analysis and first molecular characterization of Cryptosporidium spp. in horses from Rio de Janeiro, Brazil

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Federal Fluminense (UFF)-
Autor(es): dc.contributorLaboratório de Parasitologia-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorLaboratório de Toxoplasmose e outras Protozoooses-
Autor(es): dc.contributorEstrada Wenceslau José de Medeiros-
Autor(es): dc.creatorRibeiro, Daniella Sother Carvalho-
Autor(es): dc.creatorMartins, André Vianna-
Autor(es): dc.creatorLobão, Lucas Fernandes-
Autor(es): dc.creatorRibeiro, Mariana Santos-
Autor(es): dc.creatorPalmer, João Pedro Siqueira-
Autor(es): dc.creatorCorrêa, Lais Lisboa-
Autor(es): dc.creatorUchôa, Claudia Maria Antunes-
Autor(es): dc.creatorda Silva, Sidnei-
Autor(es): dc.creatorMeireles, Marcelo Vasconcelos-
Autor(es): dc.creatorAmendoeira, Maria Regina Reis-
Autor(es): dc.creatorBarbosa, Alynne da Silva-
Data de aceite: dc.date.accessioned2025-08-21T22:32:32Z-
Data de disponibilização: dc.date.available2025-08-21T22:32:32Z-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.vprsr.2021.100665-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/233851-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/233851-
Descrição: dc.descriptionAn analysis was made of the frequency of Cryptosporidium spp. in fecal samples from horses raised on farms in the Teresópolis city, state of Rio de Janeiro, Brazil, and the risk factors that favored this infection. Between 2019 and 2020, 314 samples of equine feces were collected, 287 of which came from English Thoroughbred horses and 27 from ponies. Information on the horses and their management were retrieved from a stud book and forms filled out by trainers. The fecal samples were subjected to macroscopic analysis, modified Sheather's and Lutz parasitological techniques, safranin staining, and to enzyme-linked immunosorbent assay (ELISA) for the detection of coproantigens. All the samples that tested positive by these techniques underwent partial sequence analysis of the 18S rRNA gene to characterize the protozoan species. Cryptosporidium spp. was identified in 35 (11.1%) of the samples, 34 from English Thoroughbred horses and one from a pony. Based on a logistic regression model, it was found that the presence of dogs and small ruminants on the farms, and drinking water from a spring, were significantly associated with the animals' infection by the protozoan (p < 0.05). Eight of the English Thoroughbred horse samples underwent molecular characterization, which revealed the presence of Cryptosporidium felis in one sample and Cryptosporidium parvum in seven. The seven samples containing C. parvum were subjected to gp60 gene analysis, based on which nucleotide sequences typical of the IIa family were identified, which are usually transmitted from animals to humans. In addition, the genotype IIaA15G2R1, which is considered to have the highest profile of zoonotic transmissibility, was identified in one Thoroughbred horse. This is the first study conducted in the state of Rio de Janeiro that molecularly characterized Cryptosporidium spp. in horses, and the first on the American continent to detect C. felis in the feces of these animals.-
Descrição: dc.descriptionUniversidade Federal Fluminense Instituto Biomédico Departamento de Microbiologia e Parasitologia, Rua Hernani de Mello, 101, São Domingos, Niterói-
Descrição: dc.descriptionFundação Oswaldo Cruz Instituto Nacional de Infectologia Evandro Chagas Laboratório de Parasitologia, Avenida Brasil, 4365, Manguinhos-
Descrição: dc.descriptionUniversidade Estadual Paulista Campus Araçatuba, Rua Clóvis Pestana, 793, Bairro Dona Amélia-
Descrição: dc.descriptionFundação Oswaldo Cruz Instituto Oswaldo Cruz Laboratório de Toxoplasmose e outras Protozoooses, Avenida Brasil, 4365, Manguinhos-
Descrição: dc.descriptionCentro Universitário Serra dos Órgãos Faculdade de Medicina Veterinária Campus Quinta do Paraíso Estrada Wenceslau José de Medeiros, 1045, Prata, Teresópolis-
Descrição: dc.descriptionUniversidade Estadual Paulista Campus Araçatuba, Rua Clóvis Pestana, 793, Bairro Dona Amélia-
Idioma: dc.languageen-
Relação: dc.relationVeterinary Parasitology: Regional Studies and Reports-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCryptosporidiosis-
Palavras-chave: dc.subjectGastrointestinal protozoan-
Palavras-chave: dc.subjectHorses-
Palavras-chave: dc.subjectRio de Janeiro-
Título: dc.titleDiagnosis, risk factors analysis and first molecular characterization of Cryptosporidium spp. in horses from Rio de Janeiro, Brazil-
Tipo de arquivo: dc.typelivro digital-
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