Treatment of chronic spinal cord injury in dogs using amniotic membrane-derived stem cells: Preliminary results

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorOrlandin, Jéssica Rodrigues-
Autor(es): dc.creatorGomes, Ingrid da Silva-
Autor(es): dc.creatorLeandro, Shamira de Fátima Sallum-
Autor(es): dc.creatorCagnim, Artur Fuertes-
Autor(es): dc.creatorCasals, Juliana Barbosa-
Autor(es): dc.creatorCarregaro, Adriano Bonfim-
Autor(es): dc.creatorFreitas, Silvio Henrique-
Autor(es): dc.creatorMachado, Luciana Cristina-
Autor(es): dc.creatorCastiglioni, Maria Cristina Reis-
Autor(es): dc.creatorAlves, Ana Liz Garcia-
Autor(es): dc.creatorMachado, Vânia Maria de Vasconcelos-
Autor(es): dc.creatorAmbrósio, Carlos Eduardo-
Data de aceite: dc.date.accessioned2025-08-21T22:15:57Z-
Data de disponibilização: dc.date.available2025-08-21T22:15:57Z-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.2147/SCCAA.S324184-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/233738-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/233738-
Descrição: dc.descriptionIntroduction: Intervertebral disc diseases (IVDD) represent the majority of neurological attendance and responsible for the most cases of paralysis in dogs. Treatments currently used do not show satisfactory results in patients with more severe and chronic neurological manifestations. Methods: To promote nerve and muscular recovery, as well as improve quality of life, we aimed to create a double-blind test method, associating spinal decompression surgery and allogeneic transplantation of amniotic membrane-derived stem cells (AMSCs) in dogs with chronic IVDD. Cells were characterized as fetal mesenchymal cells and safe for application. Eight animals completed the experiment: stem cell applications were made in four animals that had previously undergone an unsuccessful surgical procedure (“SC group”, n = 4); two animals were submitted to surgery, followed by applications of stem cells (“Surgery + SC”, n = 2); two other animals were submitted to surgery, followed by the application of saline solution (“Surgery + placebo”, n = 2). During the surgical procedure, a topical application was performed on the lesion and after fifteen and forty-five days another two applications were made via epidural. Animals were monitored biweekly and reassessed three months after surgery, by functional tests and magnetic resonance exams. Results: Some animals presented significant neurological improvement, such as the recovery of nociception and ability to remain on station. Despite the need further studies, until the present moment, cell therapy has been feasible and has no harmful effects on animals. Conclusion: The protocol of preclinical trial showed the association with decompressive surgery and cell transplantation in dogs with thoracolumbar IVDD proved feasible, and it was possible to observe neurological improvement after treatment. No tissue improvement through MRI was found. The double-blind test guaranteed reliability of the evaluations and results obtained that, even with a small sample size, generated satisfactory results for the animals and owners.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Veterinary Medicine Faculty of Animal Science and Food Engineering (FZEA-USP) University of São Paulo-
Descrição: dc.descriptionDepartment of Veterinary Medicine School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Veterinary Medicine School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2009/54028-8-
Descrição: dc.descriptionFAPESP: 2017/21266-0-
Formato: dc.format39-49-
Idioma: dc.languageen-
Relação: dc.relationStem Cells and Cloning: Advances and Applications-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAmnion-
Palavras-chave: dc.subjectCell therapy-
Palavras-chave: dc.subjectDiscopathy-
Palavras-chave: dc.subjectRegenerative medicine-
Palavras-chave: dc.subjectSpinal cord injury-
Título: dc.titleTreatment of chronic spinal cord injury in dogs using amniotic membrane-derived stem cells: Preliminary results-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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