A protective vaccine against the toxic activities following Brown spider accidents based on recombinant mutated phospholipases D as antigens

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Autor(es): dc.contributorUniversidade Federal do Paraná (UFPR)-
Autor(es): dc.contributorState Department of Health-
Autor(es): dc.contributorUniversidade Estadual de Ponta Grossa (UEPG)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorPolli, Nayanne Louise Costacurta-
Autor(es): dc.creatorJusta, Hanna Camara da-
Autor(es): dc.creatorAntunes, Bruno Cesar-
Autor(es): dc.creatorSilva, Thais Pereira da-
Autor(es): dc.creatorDittrich, Rosangela Locatelli-
Autor(es): dc.creatorde Souza, Giovana Scuissiatto-
Autor(es): dc.creatorWille, Ana Carolina Martins-
Autor(es): dc.creatorMatsubara, Fernando Hitomi-
Autor(es): dc.creatorMinozzo, João Carlos-
Autor(es): dc.creatorMariutti, Ricardo Barros-
Autor(es): dc.creatorArni, Raghuvir Krishnaswamy-
Autor(es): dc.creatorSenff-Ribeiro, Andrea-
Autor(es): dc.creatorVeiga, Silvio Sanches-
Autor(es): dc.creatorGremski, Luiza Helena-
Data de aceite: dc.date.accessioned2025-08-21T22:27:46Z-
Data de disponibilização: dc.date.available2025-08-21T22:27:46Z-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2021-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ijbiomac.2021.10.005-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/233692-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/233692-
Descrição: dc.descriptionAccidents involving Brown spiders are reported throughout the world. In the venom, the major toxins involved in the deleterious effects are phospholipases D (PLDs). In this work, recombinant mutated phospholipases D from three endemic species medically relevant in South America (Loxosceles intermedia, L. laeta and L. gaucho) were tested as antigens in a vaccination protocol. In such isoforms, key amino acid residues involved in catalysis, magnesium-ion coordination, and binding to substrates were replaced by Alanine (H12A-H47A, E32A-D34A and W230A). These mutations eliminated the phospholipase activity and reduced the generation of skin necrosis and edema to residual levels. Molecular modeling of mutated isoforms indicated that the three-dimensional structures, topologies, and surface charges did not undergo significant changes. Mutated isoforms were recognized by sera against the crude venoms. Vaccination protocols in rabbits using mutated isoforms generated a serum that recognized the native PLDs of crude venoms and neutralized dermonecrosis and edema induced by L. intermedia venom. Vaccination of mice prevented the lethal effects of L. intermedia crude venom. Furthermore, vaccination of rabbits prevented the cutaneous lesion triggered by the three venoms. These results indicate a great potential for mutated recombinant PLDs to be employed as antigens in developing protective vaccines for Loxoscelism.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionUniversidade Federal do Paraná-
Descrição: dc.descriptionFundação Araucária-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Cell Biology Federal University of Paraná (UFPR)-
Descrição: dc.descriptionProduction and Research Center of Immunobiological Products (CPPI) State Department of Health-
Descrição: dc.descriptionVeterinary Hospital Federal University of Paraná (UFPR)-
Descrição: dc.descriptionDepartment of Structural Molecular Biology and Genetics State University of Ponta Grossa (UEPG)-
Descrição: dc.descriptionMultiuser Center for Biomolecular Innovation Departament of Physics Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionMultiuser Center for Biomolecular Innovation Departament of Physics Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionUniversidade Federal do Paraná: 02/2020-
Descrição: dc.descriptionUniversidade Federal do Paraná: 04/2019-
Descrição: dc.descriptionFundação Araucária: 057/2017-
Descrição: dc.descriptionCNPq: 303868/2016-3-
Descrição: dc.descriptionCNPq: 408633/2018-2-
Formato: dc.format757-770-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Biological Macromolecules-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBrown spider-
Palavras-chave: dc.subjectLoxoscelism-
Palavras-chave: dc.subjectMutated phospholipases D-
Palavras-chave: dc.subjectVaccines-
Título: dc.titleA protective vaccine against the toxic activities following Brown spider accidents based on recombinant mutated phospholipases D as antigens-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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