Hypoglycemic active principles from the leaves of Bauhinia holophylla: Comprehensive phytochemical characterization and in vivo activity profile

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Geneva-
Autor(es): dc.contributorGC-
Autor(es): dc.creatorSaldanha, Luiz Leonardo-
Autor(es): dc.creatorDelgado, Aislan Quintiliano-
Autor(es): dc.creatorMarcourt, Laurence-
Autor(es): dc.creatorde Paula Camaforte, Nathalia Aparecida-
Autor(es): dc.creatorVareda, Priscilla Maria Ponce-
Autor(es): dc.creatorEbrahimi, Samad Nejad-
Autor(es): dc.creatorVilegas, Wagner-
Autor(es): dc.creatorDokkedal, Anne Lígia-
Autor(es): dc.creatorQueiroz, Emerson Ferreira-
Autor(es): dc.creatorWolfender, Jean-Luc-
Autor(es): dc.creatorBosqueiro, José Roberto-
Data de aceite: dc.date.accessioned2025-08-21T21:46:46Z-
Data de disponibilização: dc.date.available2025-08-21T21:46:46Z-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2021-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0258016-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/233585-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/233585-
Descrição: dc.descriptionBauhinia holophylla leaves, also known as pata-de-vaca, are traditionally used in Brazil to treat diabetes. Although the hypoglycemic activity of this medicinal plant has already been described, the active compounds responsible for the hypoglycemic activity have not yet been identified. To rapidly obtain two fractions in large amounts compatible with further in vivo assay, the hydroalcoholic extract of B. holophylla leaves was fractionated by Vacuum Liquid Chromatography and then purified by medium pressure liquid chromatography combined with an in vivo Glucose Tolerance Test in diabetic mice. This approach resulted in the identification of eleven compounds (1-11), including an original non-cyanogenic cyanoglucoside derivative. The structures of the isolated compounds were elucidated by nuclear magnetic resonance and high-resolution mass spectrometry. One of the major compounds of the leaves, lithospermoside (3), exhibited strong hypoglycemic activity in diabetic mice at the doses of 10 and 20 mg/kg b.w. and prevents body weight loss. The proton nuclear magnetic resonance (1H NMR) quantification revealed that the hydroalcoholic leaves extract contained 1.7% of lithospermoside (3) and 3.1% of flavonoids. The NMR analysis also revealed the presence of a high amount of pinitol (4) (9.5%), a known compound possessing in vivo hypoglycemic activity. The hypoglycemic properties of the hydroalcoholic leaves extract and the traditional water infusion extracts of the leaves of B. holophylla seem thus to be the result of the activity of three unrelated classes of compounds. Such results support to some extent the traditional use of Bauhinia holophylla to treat diabetes.-
Descrição: dc.descriptionFaculty of Sciences São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences University of Geneva-
Descrição: dc.descriptionInstitute of Pharmaceutical Sciences of Western Switzerland (ISPSO) University of Geneva-
Descrição: dc.descriptionDepartment of Phytochemistry Medicinal Plants and Drugs Research Institute Shahid Beheshti University GC, Tehran-
Descrição: dc.descriptionInstitute of Biociences São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionFaculty of Sciences São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionInstitute of Biociences São Paulo State University (UNESP), São Paulo-
Idioma: dc.languageen-
Relação: dc.relationPLoS ONE-
???dc.source???: dc.sourceScopus-
Título: dc.titleHypoglycemic active principles from the leaves of Bauhinia holophylla: Comprehensive phytochemical characterization and in vivo activity profile-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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