In vivo study of hypericin-loaded poloxamer-based mucoadhesive in situ gelling liquid crystalline precursor system in a mice model of vulvovaginal candidiasis

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorCampina Grande-
Autor(es): dc.creatorDe Araújo, Patricia Rocha-
Autor(es): dc.creatorCalixto, Giovana Maria Fioramonti-
Autor(es): dc.creatorAraújo, Victor Hugo Sousa-
Autor(es): dc.creatorSato, Mariana Rillo-
Autor(es): dc.creatorRodero, Camila Fernanda-
Autor(es): dc.creatorOshiro-Junior, João Augusto-
Autor(es): dc.creatorBauab, Taís Maria-
Autor(es): dc.creatorChorilli, Marlus-
Data de aceite: dc.date.accessioned2025-08-21T19:53:39Z-
Data de disponibilização: dc.date.available2025-08-21T19:53:39Z-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2021-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1093/mmy/myab006-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/233444-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/233444-
Descrição: dc.descriptionThe present study reports the performance of the pigment hypericin (HYP)-loaded poloxamer-based mucoadhesive in situ gelling liquid crystalline precursor system (LCPS) for the treatment of vulvovaginal candidiasis (VVC) in mice. LCPS composed of 40% of ethoxylated and propoxylated cetyl alcohol, 30% of oleic acid and cholesterol (7:1), 30% of a dispersion of 16% poloxamer 407 and 0.05% of HYP (HYP-LCPS) was prepared and characterized by polarized light microscopy (PLM), small-angle X-ray scattering (SAXS) and ex vivo permeation and retention studies across vaginal porcine mucosa were performed. In addition, the antifungal properties of the HYP-LCPS were evaluated in a murine in vivo model; for this, infected C57BL female mice groups were treated with both HYP in solution and HYP-LCPS, and after 6 days colony forming unit (CFU)/ml count was performed. PLM and SAXS confirmed that HYP-LCPS is a microemulsion situated in boundary transition region confirming its action as an LCPS. When in contact with simulated vaginal fluid, HYP-LCPS became rigid and exhibited maltase crosses and bragg peaks characteristics of lamellar phase. Ex vivo permeation and retention studies showed that HYP-LCPS provides a localized treatment on the superficial layers of porcine vaginal mucosa. HYP-LCPS induced a significant reduction in the number of CFU/ml in the mice; thus this formulation indicated it is as effective as a commercial dosage form. It was concluded that LCPS maintains the biological activity of HYP and provides an adequate drug delivery system for this lipophilic molecule at the vaginal mucosa, being a promising option in cases of VVC.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences, Araraquara-
Descrição: dc.descriptionDepartment of Biosciences Piracicaba Dental School University of Campinas - UNICAMP, Piracicaba-
Descrição: dc.descriptionParaíba State University Campina Grande-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences, Araraquara-
Descrição: dc.descriptionFAPESP: 16/11198-4-
Descrição: dc.descriptionFAPESP: 2019/10261-2-
Formato: dc.format821-827-
Idioma: dc.languageen-
Relação: dc.relationMedical Mycology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjecthypericin-
Palavras-chave: dc.subjectliquid crystalline system-
Palavras-chave: dc.subjectmurine model-
Palavras-chave: dc.subjectvulvovaginal candidiasis-
Título: dc.titleIn vivo study of hypericin-loaded poloxamer-based mucoadhesive in situ gelling liquid crystalline precursor system in a mice model of vulvovaginal candidiasis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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