Human mesenchymal stem cells seeded on the natural membrane to neurospheres for cholinergic-like neurons

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorPelé Pequeno Príncipe Institute-
Autor(es): dc.contributorFederal University of Paraná-
Autor(es): dc.contributorUniversity of Brasília-
Autor(es): dc.contributorParnaíba Delta Federal University-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Paris-
Autor(es): dc.creatorStricker, Priscila Elias Ferreira-
Autor(es): dc.creatorde Souza Dobuchak, Daiany-
Autor(es): dc.creatorIrioda, Ana Carolina-
Autor(es): dc.creatorMogharbel, Bassam Felipe-
Autor(es): dc.creatorFranco, Celia Regina Cavichiolo-
Autor(es): dc.creatorde Souza Almeida Leite, José Roberto-
Autor(es): dc.creatorde Araújo, Alyne Rodrigues-
Autor(es): dc.creatorBorges, Felipe Azevedo-
Autor(es): dc.creatorHerculano, Rondinelli Donizetti-
Autor(es): dc.creatorde Oliveira Graeff, Carlos Frederico-
Autor(es): dc.creatorChachques, Juan Carlos-
Autor(es): dc.creatorde Carvalho, Katherine Athayde Teixeira-
Data de aceite: dc.date.accessioned2025-08-21T15:37:24Z-
Data de disponibilização: dc.date.available2025-08-21T15:37:24Z-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2021-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/membranes11080598-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/233410-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/233410-
Descrição: dc.descriptionThis study aimed to differentiate human mesenchymal stem cells (hMSCs) from the human umbilical cord in cholinergic-like neurons using a natural membrane. The isolation of hMSCs from Wharton’s jelly (WJ) was carried out using “explant” and mononuclear cells by the density gradient from umbilical blood and characterized by flow cytometry. hMSCs were seeded in a natural functional biopolymer membrane to produce neurospheres. RT-PCR was performed on hMSCs and neurospheres derived from the umbilical cord. Neural precursor cells were subjected to a standard cholinergic-like neuron differentiation protocol. Dissociated neurospheres, neural precursor cells, and cholinergic-like neurons were characterized by immunocytochemistry. hMSCs were CD73+, CD90+, CD105+, CD34-and CD45-and demonstrated the trilineage differentiation. Neurospheres and their isolated cells were nestin-positive and expressed NESTIN, MAP2, ßIII-TUBULIN, GFAP genes. Neural precursor cells that were differentiated in cholinergic-like neurons expressed ßIII-TUBULIN protein and choline acetyltransferase enzyme. hMSCs seeded on the natural membrane can differentiate into neurospheres, obtaining neural precursor cells without growth factors or gene transfection before cholinergic phenotype differentiation.-
Descrição: dc.descriptionAdvanced Therapy and Cellular Biotechnology in Regenerative Medicine Department Child and Adolescent Health Research and Pequeno Príncipe Faculties Pelé Pequeno Príncipe Institute-
Descrição: dc.descriptionCell Biology Department Federal University of Paraná-
Descrição: dc.descriptionResearch Center in Applied Morphology and Immunology NuPMIA Faculty of Medicine University of Brasília-
Descrição: dc.descriptionBiodiversity and Biotechnology Research Parnaíba Delta Federal University-
Descrição: dc.descriptionFaculty of Pharmaceutics Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionPhysics Department São Paulo State University (UNESP)-
Descrição: dc.descriptionLaboratory Biosurgical Research Cardiovascular Division Pompidou Hospital University of Paris-
Descrição: dc.descriptionFaculty of Pharmaceutics Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionPhysics Department São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationMembranes-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiopolymer-
Palavras-chave: dc.subjectBlood-
Palavras-chave: dc.subjectCholinergic neurons-
Palavras-chave: dc.subjectMembrane-
Palavras-chave: dc.subjectMesenchymal stem cells-
Palavras-chave: dc.subjectUmbilical cord-
Título: dc.titleHuman mesenchymal stem cells seeded on the natural membrane to neurospheres for cholinergic-like neurons-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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