Loss of Selenoprotein Iodothyronine Deiodinase 3 Expression Correlates with Progression of Complete Hydatidiform Mole to Gestational Trophoblastic Neoplasia

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Autor(es): dc.contributorBrigham and Women’s Hospital-
Autor(es): dc.contributorHarvard Medical School-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorNew York University Langone Health-
Autor(es): dc.contributorUniversity of Texas Southwestern Medical Center-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorSt. Laurent, Jessica D.-
Autor(es): dc.creatorLin, Lawrence H.-
Autor(es): dc.creatorOwen, David M.-
Autor(es): dc.creatorMaestá, Izildinha-
Autor(es): dc.creatorCastaneda, Arnold-
Autor(es): dc.creatorHasselblatt, Kathleen T.-
Autor(es): dc.creatorGoldstein, Donald P.-
Autor(es): dc.creatorHorowitz, Neil S.-
Autor(es): dc.creatorBerkowitz, Ross S.-
Autor(es): dc.creatorElias, Kevin M.-
Data de aceite: dc.date.accessioned2025-08-21T16:15:30Z-
Data de disponibilização: dc.date.available2025-08-21T16:15:30Z-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2022-05-01-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s43032-021-00634-y-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/233158-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/233158-
Descrição: dc.descriptionTo investigate if differences in imprinting at tropho-microRNA (miRNA) genomic clusters can distinguish between pre-gestational trophoblastic neoplasia cases (pre-GTN) and benign complete hydatidiform mole (CHM) cases at the time of initial uterine evacuation. miRNA sequencing was performed on frozen tissue from 39 CHM cases including 9 GTN cases. DIO3, DLK1, RTL1, and MEG 3 mRNA levels were assessed by qRT-PCR. Protein abundance was assessed by Western blot for DIO3, DLK1, and RTL1. qRT-PCR and Western blot were performed for selenoproteins and markers of oxidative stress. Immunohistochemistry (IHC) was performed for DIO3 on an independent validation set of clinical samples (n = 42) and compared to normal placenta controls across gestational ages. Relative expression of the 14q32 miRNA cluster was lower in pre-GTN cases. There were no differences in protein abundance of DLK1 or RTL1. Notably, there was lower protein expression of DIO3 in pre-GTN cases (5-fold, p < 0.03). There were no differences in mRNA levels of DIO3, DLK1, RTL1 or MEG 3. mRNA levels were higher in all CHM cases compared to normal placenta. IHC showed syncytiotrophoblast-specific DIO3 immunostaining in benign CHM cases and normal placenta, while pre-GTN cases of CHM lacked DIO3 expression. We describe two new biomarkers of pre-GTN CHM cases: decreased 14q32 miRNA expression and loss of DIO3 expression by IHC. Differences in imprinting between benign CHM and pre-GTN cases may provide insight into the fundamental development of CHM.-
Descrição: dc.descriptionDivision of Gynecologic Oncology Department of Obstetrics and Gynecology Brigham and Women’s Hospital, 75 Francis St.-
Descrição: dc.descriptionNew England Trophoblastic Disease Center Division of Gynecologic Oncology Department of Obstetrics Gynecology and Reproductive Biology Brigham and Women’s Hospital Dana-Farber Cancer Institute Harvard Medical School, 75 Francis St-
Descrição: dc.descriptionUniversity of Sao Paulo Trophoblastic Disease Center University of Sao Paulo Medical School-
Descrição: dc.descriptionDepartment of Pathology New York University Langone Health-
Descrição: dc.descriptionDepartment of Obstetrics and Gynecology University of Texas Southwestern Medical Center-
Descrição: dc.descriptionBotucatu Trophoblastic Disease Center Clinical Hospital of Botucatu Medical School Sao Paulo State University Sao Paulo State University-UNESP, Botucatu-
Descrição: dc.descriptionBotucatu Trophoblastic Disease Center Clinical Hospital of Botucatu Medical School Sao Paulo State University Sao Paulo State University-UNESP, Botucatu-
Idioma: dc.languageen-
Relação: dc.relationReproductive Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectComplete hydatidiform mole-
Palavras-chave: dc.subjectIodothyronine deiodinase 3-
Palavras-chave: dc.subjectMicroRNA-
Palavras-chave: dc.subjectPlacenta-
Título: dc.titleLoss of Selenoprotein Iodothyronine Deiodinase 3 Expression Correlates with Progression of Complete Hydatidiform Mole to Gestational Trophoblastic Neoplasia-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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