Can centrifugation force compromise the plasmatic membrane, acrosome and DNA integrity of goat spermatozoa?

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Santo Amaro-
Autor(es): dc.contributorUniversidade Severino Sombra-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorVetsemen®-Análise de sêmen para Inseminação Artificial-
Autor(es): dc.creatorCrespilho, André Maciel-
Autor(es): dc.creatorBosco, Karinne Ávila-
Autor(es): dc.creatorDell’aqua, Camila de Paula Freitas-
Autor(es): dc.creatorSegabinazzi, Lorenzo Garrido-
Autor(es): dc.creatorPapa, Frederico Ozanam-
Autor(es): dc.creatorBrás, Karoline Maria Gil-
Autor(es): dc.creatorGomes, Gustavo Mendes-
Autor(es): dc.creatorPeixoto Junior, Kleber da Cunha-
Data de aceite: dc.date.accessioned2025-08-21T18:37:27Z-
Data de disponibilização: dc.date.available2025-08-21T18:37:27Z-
Data de envio: dc.date.issued2022-04-30-
Data de envio: dc.date.issued2022-04-30-
Data de envio: dc.date.issued2018-10-24-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.11606/issn.1678-4456.bjvras.2018.145873-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/232842-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/232842-
Descrição: dc.descriptionProtocols for cooling or freezing goat semen usually recommend centrifugation for seminal plasma removal. However, little is known about the effect of this process on goat sperm viability and functionality. The present study evaluated the effects of centrifugation force on the plasma membrane, acrosomes, and DNA integrity of goat semen. Four ejaculates from each of the four different Anglo Nubian male goats were used. Semen samples were obtained using artificial vagina, and immediately after collection, ejaculates were diluted using Ringer’s sodium lactate solution and split into three groups: Control (CG, without centrifugation), G1 (centrifugation 600 x g/10 min), G2 (centrifugation 1200 x g/10 min). After centrifugation, seminal plasma was removed, the sperm pellets were resuspended using Tris-egg yolk extender (80 x 106 spermatozoa/mL) and the sperm morphology was analyzed. Samples were cooled at 5°C for 5, 24, 36, and 48 h and then sperm plasma membrane and acrosome integrity (PMAI, %) and sperm DNA fragmentation index (SDF, %) were evaluated at each time-point, using a flow cytometer. Additionally, sperm movement was determined using computer semen analysis (CASA) after 5, 24, and 48 h of refrigeration period. The semen centrifugation did not induce additional sperm morphology defect or reduction in sperm kinetics in the experimental groups. Differences were not observed (p > 0.05) in PMAI and SDF among different groups, in any of each time-point of the cooling process. In conclusion, centrifugation, even at high speeds, did not affect goat sperm integrity and functionality when submitted to refrigeration process.-
Descrição: dc.descriptionUniversidade Santo Amaro-
Descrição: dc.descriptionUniversidade Severino Sombra-
Descrição: dc.descriptionUniversidade Estadual Paulista “Júlio de Mesquita Filho” Faculdade de Medicina Veterinária e Zootecnia-
Descrição: dc.descriptionVetsemen®-Análise de sêmen para Inseminação Artificial-
Descrição: dc.descriptionUniversidade Estadual Paulista “Júlio de Mesquita Filho” Faculdade de Medicina Veterinária e Zootecnia-
Idioma: dc.languageen-
Relação: dc.relationBrazilian Journal of Veterinary Research and Animal Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCentrifugation-
Palavras-chave: dc.subjectGoat-
Palavras-chave: dc.subjectIntegrity-
Palavras-chave: dc.subjectSemen-
Palavras-chave: dc.subjectViability-
Título: dc.titleCan centrifugation force compromise the plasmatic membrane, acrosome and DNA integrity of goat spermatozoa?-
Título: dc.titleA força de centrifugação pode comprometer a integridade de membrana plasmática, acrossomal e DNA de espermatozoides caprinos?-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.