Cloning and expression of the nucleoprotein gene (NP) of newcastle disease virus (NDV) in Escherichia coli for immunodiagnosis application

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorSilva, Ketherson Rodrigues-
Autor(es): dc.creatorCosta Mello GonçAlves, Mariana-
Autor(es): dc.creatorde Oliveira, Elisabete Schirato-
Autor(es): dc.creatorFernando, Filipe Santos-
Autor(es): dc.creatorde Silva Montassier, Maria Fatima-
Autor(es): dc.creatorFernandes, Camila Cesario-
Autor(es): dc.creatorde Feres Tamanine, Maria Lourdes-
Autor(es): dc.creatorBorzi, Mariana Monezi-
Autor(es): dc.creatordos Santos, Romeu Moreira-
Autor(es): dc.creatorde Mendonca, Andre Oliveira-
Autor(es): dc.creatorReischak, Dilmara-
Autor(es): dc.creatorPaulillo, Antonio Carlos-
Autor(es): dc.creatorMontassier, Helio Jose-
Data de aceite: dc.date.accessioned2025-08-21T17:38:08Z-
Data de disponibilização: dc.date.available2025-08-21T17:38:08Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2014-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3923/ijps.2014.473.479-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/232332-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/232332-
Descrição: dc.descriptionThe nucleocapsid protein (NP) of newcastle disease virus (NDV) is an important antigen to develop a serologic assay on account of its highly conserved sequences and high immunogenicity. This study aimed at expressing the gene of the NP of NDV in a heterologous system (Escherichia coli), using the appropriate vector. The NDV-NP protein was expressed as a fusion recombinant protein containing SUMO peptide and poly-histidine tags. This recombinant nucleocapsid protein (rNP) was expressed in a soluble form which was easily purified and showed the ability to react with chicken anti-NDV polyclonal antibodies. An indirect ELISA method based on the adsorption of an antigen composed by NP (rNP-NDV-ELISA) was developed. By comparing this rNP-NDV-ELISA with haemagglutination-inhibition test (HI) high and significant correlation with the HI (r = 0.83) was found. In addition to, high sensitivity (88.9%), specificity (95.5%), accuracy (90.4%) and agreement (0.85) were obtained. In conclusion the results indicated that the cloning and expression procedures used in this study provided a rNP that shared the major epitopes with the homologous viral protein and has the potential to be applied in ELISA for the immunodiagnosis of the important newcastle disease.-
Descrição: dc.descriptionUniv Estadual Paulista-
Descrição: dc.descriptionUniv Estadual Paulista-
Formato: dc.format473-479-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Poultry Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCloning of NDV-NP gene-
Palavras-chave: dc.subjectELISA-
Palavras-chave: dc.subjectExpression-
Palavras-chave: dc.subjectImmunodiagnosis Application-
Palavras-chave: dc.subjectNewcastle disease virus-
Palavras-chave: dc.subjectRecombinant nucleocapsid protein-
Palavras-chave: dc.subjectSUMO-
Título: dc.titleCloning and expression of the nucleoprotein gene (NP) of newcastle disease virus (NDV) in Escherichia coli for immunodiagnosis application-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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