Fecundação in Vitro de Ovócitos Bovinos com Sêmen Submetido a Diferentes Diluidores

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorCoelho, Lia De Alencar-
Autor(es): dc.creatorEsper, César Roberto-
Autor(es): dc.creatorGarcia, Joaquim Mansano-
Autor(es): dc.creatorVantini, Roberta-
Autor(es): dc.creatorAlmeida Jr., Ivo Luis-
Data de aceite: dc.date.accessioned2025-08-21T20:44:26Z-
Data de disponibilização: dc.date.available2025-08-21T20:44:26Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2000-03-01-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/231738-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/231738-
Descrição: dc.descriptionThis study investigated the effect of semen extender on in vitro development of bovine oocytes after in vitro maturation and fertilization. The ejaculate from one bull was divided in three parts which were diluted with three different extenders: Egg yolk/Lactose (EYL), Egg yolk/Citrate (EYC) and Egg yolk/Tris (EYT). Samples of semen diluted with these extenders were frozen in liquid nitrogen and, after thawing, were filtered by discontinuous Percoll gradient. The sperm concentration was adjusted to 10× 106 cells/mL and the sperm capacitation was induced with 10 μg/mL of heparin. After 24 hours of culture for in vitro maturation, the oocytes, aspirated from ovarian follicles, were inseminated with semen diluted in TALP medium and 48 h after the zigotes were transferred into droplets prepared with TCM-199 medium containing 5% of bovine fetal serum, 5% of estrous cow serum and bovine oviduct epithelial cells in suspension under silicon oil and were further cultured of 9 days. All the cultures were performed at 38.5°C in 5% CO 2 in air. Data were analyzed by chi-square analysis. There was a significant difference in terms of cleavage rate (CR) with means of 66.0, 69.3 and 54.4% for EYL, EYC and EYT, respectively. There were no differences in terms of the morulae/blastocysts and hatching rates. These results suggest that the semen extender had no effect on in vitro development of bovine, even the CR have been affected.-
Descrição: dc.descriptionDepto. de Zootecnia Fac. Zootecnia e Eng. de Alimentos FZEA/USP-
Descrição: dc.descriptionFaculdade Cie. Agrarias Veterinarias FCAV/UNESP-
Descrição: dc.descriptionTecn. de Lab. da FCAV/UNESP-
Descrição: dc.descriptionFaculdade Cie. Agrarias Veterinarias FCAV/UNESP-
Descrição: dc.descriptionTecn. de Lab. da FCAV/UNESP-
Formato: dc.format397-402-
Idioma: dc.languagept_BR-
Relação: dc.relationRevista Brasileira de Zootecnia-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBovine-
Palavras-chave: dc.subjectCleavage rate-
Palavras-chave: dc.subjectEmbryo-
Palavras-chave: dc.subjectIn vitro fertilization-
Palavras-chave: dc.subjectSemen extender-
Título: dc.titleFecundação in Vitro de Ovócitos Bovinos com Sêmen Submetido a Diferentes Diluidores-
Título: dc.titleIn Vitro Fertilization of Bovine Oocytes with Semen Submitted to Different Extenders-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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