Viral load is associated with mitochondrial dysfunction and altered monocyte phenotype in acute severe SARS-CoV-2 infection

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Autor(es): dc.contributorUniversidade Federal de Ciências da Saúde de Porto Alegre-
Autor(es): dc.contributorUniversidade Federal de Goiás (UFG)-
Autor(es): dc.contributorUniversidade Federal do Pará (UFPA)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorHospital Moinhos de Vento-
Autor(es): dc.contributorJohns Hopkins School of Medicine-
Autor(es): dc.creatorRomão, Pedro RT-
Autor(es): dc.creatorTeixeira, Paula C-
Autor(es): dc.creatorSchipper, Lucas-
Autor(es): dc.creatorda Silva, Igor-
Autor(es): dc.creatorSantana Filho, Paulo-
Autor(es): dc.creatorJúnior, Luiz Carlos Rodrigues-
Autor(es): dc.creatorPeres, Alessandra-
Autor(es): dc.creatorGonçalves da Fonseca, Simone-
Autor(es): dc.creatorChagas Monteiro, Marta-
Autor(es): dc.creatorLira, Fabio S-
Autor(es): dc.creatorAndrey Cipriani Frade, Marco-
Autor(es): dc.creatorComerlato, Juliana-
Autor(es): dc.creatorComerlato, Carolina-
Autor(es): dc.creatorSant'Anna, Fernando Hayashi-
Autor(es): dc.creatorBessel, Marina-
Autor(es): dc.creatorAbreu, Celina Monteiro-
Autor(es): dc.creatorWendland, Eliana M-
Autor(es): dc.creatorDorneles, Gilson P-
Data de aceite: dc.date.accessioned2025-08-21T18:52:47Z-
Data de disponibilização: dc.date.available2025-08-21T18:52:47Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.intimp.2022.108697-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/231651-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/231651-
Descrição: dc.descriptionMonocytes play a major role in the initial innate immune response to SARS-CoV-2. Although viral load may correlate with several clinical outcomes in COVID-19, much less is known regarding their impact on innate immune phenotype. We evaluated the monocyte phenotype and mitochondrial function in severe COVID-19 patients (n = 22) with different viral burden (determined by the median of viral load of the patients) at hospital admission. Severe COVID-19 patients presented lower frequency of CD14 + CD16- classical monocytes and CD39 expression on CD14 + monocytes, and higher frequency of CD14 + CD16 + intermediate and CD14-CD16 + nonclassical monocytes as compared to healthy controls independently of viral load. COVID-19 patients with high viral load exhibited increased GM-CSF, PGE-2 and lower IFN-α as compared to severe COVID-19 patients with low viral load (p < 0.05). CD14 + monocytes of COVID-19 patients with high viral load presented higher expression of PD-1 but lower HLA-DR on the cell surface than severe COVID-19 patients with low viral load. All COVID-19 patients presented decreased monocyte mitochondria membrane polarization, but high SARS-CoV-2 viral load was associated with increased mitochondrial reactive oxygen species. In this sense, higher viral load induces mitochondrial reactive oxygen species generation associated with exhaustion profile in CD14 + monocytes of severe COVID-19 patients. Altogether, these data shed light on new pathological mechanisms involving SARS-CoV-2 viral load on monocyte activation and mitochondrial function, which were associated with COVID-19 severity.-
Descrição: dc.descriptionLaboratory of Cellular and Molecular Immunology Universidade Federal de Ciências da Saúde de Porto Alegre-
Descrição: dc.descriptionGraduate Program in Health Sciences Universidade Federal de Ciências da Saúde de Porto Alegre-
Descrição: dc.descriptionGraduate Program in Biosciences Universidade Federal de Ciências da Saúde de Porto Alegre-
Descrição: dc.descriptionInstitute of Tropical Pathology and Public Health Universidade Federal de Goiás-
Descrição: dc.descriptionGraduate Program in Pharmaceutical Science Health Science Institute Federal University of Pará/UFPA-
Descrição: dc.descriptionExercise and Immunometabolism Research Group Postgraduation Program in Movement Sciences Department of Physical Education Universidade Estadual Paulista (UNESP), SP-
Descrição: dc.descriptionDermatology Division Department of Medical Clinics Ribeirão Preto Medical School University of São Paulo, São Paulo-
Descrição: dc.descriptionHospital Moinhos de Vento-
Descrição: dc.descriptionDepartment of Molecular and Comparative Pathobiology Johns Hopkins School of Medicine-
Descrição: dc.descriptionGraduate Program in Pediatrics Universidade Federal de Ciências da Saúde de Porto Alegre-
Descrição: dc.descriptionExercise and Immunometabolism Research Group Postgraduation Program in Movement Sciences Department of Physical Education Universidade Estadual Paulista (UNESP), SP-
Idioma: dc.languageen-
Relação: dc.relationInternational Immunopharmacology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCD39-
Palavras-chave: dc.subjectCOVID-19-
Palavras-chave: dc.subjectHLA-DR-
Palavras-chave: dc.subjectImmune checkpoints-
Palavras-chave: dc.subjectPD-1-
Palavras-chave: dc.subjectReactive oxygen species-
Título: dc.titleViral load is associated with mitochondrial dysfunction and altered monocyte phenotype in acute severe SARS-CoV-2 infection-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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