Telmisartan impairs the in vitro osteogenic differentiation of mesenchymal stromal cells from spontaneously hypertensive male rats

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorBalera Brito, Victor Gustavo-
Autor(es): dc.creatorPatrocinio, Mariana Sousa-
Autor(es): dc.creatorAlves Barreto, Ayná Emanuelli-
Autor(es): dc.creatorTfaile Frasnelli, Sabrina Cruz-
Autor(es): dc.creatorLara, Vanessa Soares-
Autor(es): dc.creatorSantos, Carlos Ferreira-
Autor(es): dc.creatorPenha Oliveira, Sandra Helena-
Data de aceite: dc.date.accessioned2025-08-21T15:43:45Z-
Data de disponibilização: dc.date.available2025-08-21T15:43:45Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-12-04-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ejphar.2021.174609-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/231545-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/231545-
Descrição: dc.descriptionTelmisartan (TELM) is an angiotensin II (Ang II) type 1 receptor (Agtr1) antagonist, with partial agonism for Pparg, and has been shown to affect bone metabolism. Therefore, the aim of this study was to investigate the effects of TELM in the in vitro osteogenic differentiation of bone marrow-derived mesenchymal stromal cells (BMSC) from spontaneously hypertensive rats (SHRs). BMSC were obtained from male SHR, and the osteogenic medium (OM) was added to the cells concomitantly with TELM (0.005, 0.05, and 0.5 μM). Undifferentiated BMSC, in control medium (CM), showed an increased viability, while the addition of OM reduced this parameter, and TELM did not show cytotoxicity in the concentrations used. BMSC in OM had an alkaline phosphatase (ALP) activity peak at d10, which decreased at d14 and d21, and TELM reduced ALP at d10 in a dose-dependent manner. Mineralization was observed in the OM at d14, which intensified at d21, but was inhibited by TELM. Agtr1b was increased in the OM, and TELM inhibited its expression. TELM reduced Opn, Ocn, and Bsp and increased Pparg expression, and at the higher concentration TELM also increased the expression of adipogenic markers, Fabp4 and Adipoq. In addition, TELM 0.5 μM increased Irs1 and Glut4, insulin and glucose metabolism markers, known to be regulated by Pparg and to be related to adipogenic phenotype. Our data shows that TELM inhibited the osteogenic differentiation and mineralization of SHR BMSC, by favoring an adipogenic prone phenotype due to Pparg upregulation.-
Descrição: dc.descriptionDepartment of Basic Sciences São Paulo State University (UNESP) School of Dentistry-
Descrição: dc.descriptionMulticenter Postgraduate Program in Physiological Sciences Brazilian Society of Physiology São Paulo State University (UNESP) School of Dentistry-
Descrição: dc.descriptionDepartment of Stomatology Bauru School of Dentistry University of São Paulo (USP)-
Descrição: dc.descriptionDepartment of Biological Science Bauru School of Dentistry University of São Paulo (USP)-
Descrição: dc.descriptionDepartment of Basic Sciences São Paulo State University (UNESP) School of Dentistry-
Descrição: dc.descriptionMulticenter Postgraduate Program in Physiological Sciences Brazilian Society of Physiology São Paulo State University (UNESP) School of Dentistry-
Idioma: dc.languageen-
Relação: dc.relationEuropean Journal of Pharmacology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBone marrow-derived mesenchymal stromal cell-
Palavras-chave: dc.subjectHypertension-
Palavras-chave: dc.subjectOsteogenic differentiation-
Palavras-chave: dc.subjectSpontaneously hypertensive rats-
Palavras-chave: dc.subjectTelmisartan-
Título: dc.titleTelmisartan impairs the in vitro osteogenic differentiation of mesenchymal stromal cells from spontaneously hypertensive male rats-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.