Master regulators of epithelial-mesenchymal transition and wnt signaling pathways in juvenile nasopharyngeal angiofibromas

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorA.C. Camargo Cancer Center-
Autor(es): dc.contributorSir Mortimer B. Davis-Jewish General Hospital-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorPaulista University—UNIP-
Autor(es): dc.contributorUniversity Hospital of Southern Denmark-
Autor(es): dc.contributorUniversity of Southern Denmark-
Autor(es): dc.creatorCalanca, Naiade-
Autor(es): dc.creatorBinato, Sara Martoreli Silveira-
Autor(es): dc.creatorSilva, Sabrina Daniela da-
Autor(es): dc.creatorBrentani, Helena Paula-
Autor(es): dc.creatorSennes, Luiz Ubirajara-
Autor(es): dc.creatorPinto, Clóvis Antonio Lopes-
Autor(es): dc.creatorDomingues, Maria Aparecida Custódio-
Autor(es): dc.creatorFonseca-Alves, Carlos Eduardo-
Autor(es): dc.creatorRainho, Claudia Aparecida-
Autor(es): dc.creatorRogatto, Silvia Regina-
Data de aceite: dc.date.accessioned2025-08-21T21:25:00Z-
Data de disponibilização: dc.date.available2025-08-21T21:25:00Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/biomedicines9091258-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/231519-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/231519-
Descrição: dc.descriptionJuvenile nasopharyngeal angiofibroma (JNA) is a rare fibrovascular benign tumor showing an invasive growth pattern and affecting mainly male adolescents. We investigated the role of epithelial–mesenchymal transition (EMT) and WNT signaling pathways in JNA. Gene expression profiles using nine JNA paired with four inferior nasal turbinate samples were interrogated using a customized 2.3K microarray platform containing genes mainly involved in EMT and WNT/PI3K pathways. The expression of selected genes (BCL2, CAV1, CD74, COL4A2, FZD7, ING1, LAMB1, and RAC2) and proteins (BCL2, CAV1, CD74, FZD7, RAF1, WNT5A, and WNT5B) was investigated by RT-qPCR (28 cases) and immunohistochemistry (40 cases), respectively. Among 104 differentially expressed genes, we found a significantly increased expression of COL4A2 and LAMB1 and a decreased expression of BCL2 and RAC2 by RT-qPCR. The immunohistochemistry analysis revealed a low expression of BCL2 and a negative to moderate expression of FZD7 in most samples, while increased CAV1 and RAF1 expression were detected. Moderate to strong CD74 protein expression was observed in endothelial and inflammatory cells. A significant number of JNAs (78%) presented reduced WNT5A and increased WNT5B expression. Overall, the transcript and protein profile indicated the involvement of EMT and WNT pathways in JNA. These candidates are promising druggable targets for treating JNA.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionInternational Research Center CIPE A.C. Camargo Cancer Center-
Descrição: dc.descriptionDepartment of Otolaryngology—Head and Neck Surgery McGill University Sir Mortimer B. Davis-Jewish General Hospital-
Descrição: dc.descriptionDepartment of Psychiatry LIM23 (FMUSP) University of Sao Paulo (USP)-
Descrição: dc.descriptionDepartment of Otorhinolaryngology FMUSP University of São Paulo (USP)-
Descrição: dc.descriptionDepartment of Pathology A.C. Camargo Cancer Center-
Descrição: dc.descriptionDepartment of Pathology Faculty of Medicine São Paulo State University (UNESP)-
Descrição: dc.descriptionInstitute of Health Sciences Paulista University—UNIP-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Anesthesiology Sao Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Clinical Genetics University Hospital of Southern Denmark-
Descrição: dc.descriptionInstitute of Regional Health Research University of Southern Denmark-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Pathology Faculty of Medicine São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Anesthesiology Sao Paulo State University (UNESP)-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionCAPES: 88887.310463/2018-00-
Descrição: dc.descriptionCAPES: 88887.374335/2019-00-
Idioma: dc.languageen-
Relação: dc.relationBiomedicines-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectEpithelial–mesenchymal transition-
Palavras-chave: dc.subjectGene expression profile-
Palavras-chave: dc.subjectJuvenile nasopharyngeal angiofibroma-
Palavras-chave: dc.subjectTumor microenvironment-
Palavras-chave: dc.subjectTumor signaling pathways-
Palavras-chave: dc.subjectWNT pathway-
Título: dc.titleMaster regulators of epithelial-mesenchymal transition and wnt signaling pathways in juvenile nasopharyngeal angiofibromas-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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