Comparison of two different methods for detecting periodontal pathogenic bacteria

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorNove de Julho University-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorBedran, Telma Blanca Lombardo-
Autor(es): dc.creatorde Oliveira, Guilherme José Pimentel Lopes-
Autor(es): dc.creatorSpolidorio, Luís Carlos-
Autor(es): dc.creatorCirelli, Joni Augusto-
Autor(es): dc.creatorSpolidorio, Denise Palomari-
Data de aceite: dc.date.accessioned2025-08-21T17:15:55Z-
Data de disponibilização: dc.date.available2025-08-21T17:15:55Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2016-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.20396/bjos.v15i3.8649599-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/231408-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/231408-
Descrição: dc.descriptionAim: To perform a comparative analysis between two methods for detecting Porphyromonas gingivalis, Tannerella forsythia and Porphyromonas endodontalis in periodontal plaque samples.Methods: The study sample consisted of twenty systemically healthy patients showing generalized chronic periodontitis. The subgingival samples for microbiological analysis were collected before (baseline) and 60 days after a basic periodontal therapy from 30 non-adjacent affected sites (Probing Depth (PD): 5-7 mm, Clinical Attachment Loss (CAL) ≥ 5 mm, positive for Bleeding on Probing (BOP)). Microbiological analysis was performed by PCR and qPCR. To allow a comparative analysis between both methods, qPCR was divided in three different scores (score 2: presence of more than 100 bacteria; score 1: presence of 10-100 bacteria, and score 0: absence of bacteria), in accordance to DNA quantity, while for PCR two scores were assigned: presence or absence of bacteria. Results: qPCR demonstrated higher sensitivity in the detection of these pathogens compared with PCR when scores 1 and 2 were considered positive. However, when only score 2 was considered positive, PCR and qPCR showed better agreement. Conclusions: qPCR demonstrated higher sensitivity than conventional PCR for detection of low numbers of microorganisms and can be useful for the quantification of periodontopathogens.-
Descrição: dc.descriptionDepartment of Dentistry Nove de Julho University-
Descrição: dc.descriptionDepartment of Oral Diagnosis and Surgery Araraquara Dental School State University of São Paulo-
Descrição: dc.descriptionDepartment of Physiology and Pathology Araraquara Dental School State University of São Paulo-
Formato: dc.format166-172-
Idioma: dc.languageen-
Relação: dc.relationBrazilian Journal of Oral Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBacteria-
Palavras-chave: dc.subjectPeriodontal diseases-
Palavras-chave: dc.subjectPolymerase chain reaction-
Título: dc.titleComparison of two different methods for detecting periodontal pathogenic bacteria-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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