Expression signatures of DNA repair genes correlate with survival prognosis of astrocytoma patients

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorRegional Blood Center of Ribeirão Preto and Center for Cell-Based Therapy—CEPID/FAPESP-
Autor(es): dc.contributorNational Institute of Science and Technology in Stem cell and Cell Therapy-
Autor(es): dc.creatorde Sousa, Juliana Ferreira-
Autor(es): dc.creatorTorrieri, Raul-
Autor(es): dc.creatorSerafim, Rodolfo Bortolozo-
Autor(es): dc.creatorDi Cristofaro, Luis Fernando Macedo-
Autor(es): dc.creatorEscanfella, Fábio Dalbon-
Autor(es): dc.creatorRibeiro, Rodrigo-
Autor(es): dc.creatorZanette, Dalila Lucíola-
Autor(es): dc.creatorPaçó-Larson, Maria Luisa-
Autor(es): dc.creatorda Silva, Wilson Araujo-
Autor(es): dc.creatorTirapelli, Daniela Pretti da Cunha-
Autor(es): dc.creatorNeder, Luciano-
Autor(es): dc.creatorCarlotti, Carlos Gilberto-
Autor(es): dc.creatorValente, Valeria-
Data de aceite: dc.date.accessioned2025-08-21T17:37:51Z-
Data de disponibilização: dc.date.available2025-08-21T17:37:51Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2017-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1177/1010428317694552-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/231396-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/231396-
Descrição: dc.descriptionAstrocytomas are the most common primary brain tumors. They are very resistant to therapies and usually progress rapidly to high-grade lesions. Here, we investigated the potential role of DNA repair genes in astrocytoma progression and resistance. To this aim, we performed a polymerase chain reaction array-based analysis focused on DNA repair genes and searched for correlations between expression patters and survival prognoses. We found 19 genes significantly altered. Combining these genes in all possible arrangements, we found 421 expression signatures strongly associated with poor survival. Importantly, five genes (DDB2, EXO1, NEIL3, BRCA2, and BRIP1) were independently correlated with worse prognoses, revealing single-gene signatures. Moreover, silencing of EXO1, which is remarkably overexpressed, promoted faster restoration of double-strand breaks, while NEIL3 knockdown, also highly overexpressed, caused an increment in DNA damage and cell death after irradiation of glioblastoma cells. These results disclose the importance of DNA repair pathways for the maintenance of genomic stability of high-grade astrocytomas and suggest that EXO1 and NEIL3 overexpression confers more efficiency for double-strand break repair and resistance to reactive oxygen species, respectively. Thereby, we highlight these two genes as potentially related with tumor aggressiveness and promising candidates as novel therapeutic targets.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Clinical Analysis Faculty of Pharmaceutical Sciences of Araraquara University of São Paulo State-
Descrição: dc.descriptionDepartment of Cellular and Molecular Biology Ribeirão Preto Medical School University of São Paulo (USP)-
Descrição: dc.descriptionFAEPA Center for Medical Genomics (CMG) of the Clinical Hospital Ribeirão Preto Medical School University of São Paulo (USP)-
Descrição: dc.descriptionDepartment of Genetics Ribeirão Preto Medical School University of São Paulo (USP)-
Descrição: dc.descriptionRegional Blood Center of Ribeirão Preto and Center for Cell-Based Therapy—CEPID/FAPESP-
Descrição: dc.descriptionNational Institute of Science and Technology in Stem cell and Cell Therapy-
Descrição: dc.descriptionCenter for Integrative Systems Biology (CISBi) NAP/USP-
Descrição: dc.descriptionDepartment of Surgery and Anatomy Ribeirão Preto Medical School University of São Paulo (USP)-
Descrição: dc.descriptionDepartment of Pathology Ribeirão Preto Medical School University of São Paulo (USP)-
Descrição: dc.descriptionFAPESP: 2013/13465-1-
Idioma: dc.languageen-
Relação: dc.relationTumor Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectastrocytoma-
Palavras-chave: dc.subjectDNA repair-
Palavras-chave: dc.subjectgenomic instability-
Palavras-chave: dc.subjectglioblastoma-
Palavras-chave: dc.subjecttumor progression-
Título: dc.titleExpression signatures of DNA repair genes correlate with survival prognosis of astrocytoma patients-
Tipo de arquivo: dc.typelivro digital-
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