Production of β-galactosidase by Trichoderma reesei FTKO-39 in wheat bran: Partial purification of two isozymes

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorInstitute of Chemistry-
Autor(es): dc.contributorInstitute of Biosciences-
Autor(es): dc.contributorSchool of Pharmaceutical Sciences-
Autor(es): dc.creatorAdalberto, P. R.-
Autor(es): dc.creatorMassabni, A. C.-
Autor(es): dc.creatorGoulart, A. J.-
Autor(es): dc.creatorContiero, J.-
Autor(es): dc.creatorCarmona, E. C.-
Autor(es): dc.creatorCardello, L.-
Autor(es): dc.creatorMonti, R.-
Data de aceite: dc.date.accessioned2025-08-21T23:12:07Z-
Data de disponibilização: dc.date.available2025-08-21T23:12:07Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2006-05-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1385/ABAB:133:2:163-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/231012-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/231012-
Descrição: dc.descriptionTrichoderma reesei FTKO-39 grown at 35°C for 5 d on wheat bran supplemented with MgCl2 and lactose as the carbon source produced two isozymes of β-galactosidase: BGT I and BGT II. These isozymes were partially purified on a DEAE-Trisacryl column. Both BGT I and BGT II fractions exhibited optimum activity at 65°C, but the pH optima were 4.0 and 6.5, respectively. The isozymes also showed similar thermal stability. However, BGT I was more stable than BGT II in a pH range of 3.0-10.0. At least two different β-galactosidases are produced by T. reesei, as revealed by the two bands seen on a 6% polyacrylamide gel stained for activity. Copyright © 2006 by Humana Press Inc. All rights of any nature whatsoever reserved.-
Descrição: dc.descriptionDepartment of General Inorganic and Chemistry Institute of Chemistry, Rua Prof. Francisco Degni, s/n, 14800-900, Araraquara, SP-
Descrição: dc.descriptionDepartment of Biochemistry and Technological Chemistry Institute of Chemistry, Rua Prof. Francisco Degni, s/n, 14800-900, Araraquara, SP-
Descrição: dc.descriptionDepartment of Biochemistry and Microbiology Institute of Biosciences, Avenida 24-A, 1515, 13506-900, Rio Claro, SP-
Descrição: dc.descriptionDepartment of Food and Nutrition School of Pharmaceutical Sciences, Rodovia Araraquara-Jaú Km1, 14801-902, Araraquara, SP-
Formato: dc.format163-170-
Idioma: dc.languageen-
Relação: dc.relationApplied Biochemistry and Biotechnology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectβ-Galactosidase-
Palavras-chave: dc.subjectIsozymes-
Palavras-chave: dc.subjectPartial purification-
Palavras-chave: dc.subjectSemisolid fermentation-
Palavras-chave: dc.subjectTrichoderma reesei-
Título: dc.titleProduction of β-galactosidase by Trichoderma reesei FTKO-39 in wheat bran: Partial purification of two isozymes-
Tipo de arquivo: dc.typelivro digital-
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