Protease-activated receptor-2 activation: A major role in the pathogenesis of Porphyromonas gingivalis infection

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Autor(es): dc.contributorUniversity of Calgary-
Autor(es): dc.contributorUniversity of Toronto-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversity Hospital-
Autor(es): dc.contributorJohnson and Johnson Pharmaceutical Research and Development-
Autor(es): dc.creatorHolzhausen, Marinella-
Autor(es): dc.creatorSpolidorio, Luis Carlos-
Autor(es): dc.creatorEllen, Richard P.-
Autor(es): dc.creatorJobin, Marie-Claude-
Autor(es): dc.creatorSteinhoff, Martin-
Autor(es): dc.creatorAndrade-Gordon, Patricia-
Autor(es): dc.creatorVergnolle, Nathalie-
Data de aceite: dc.date.accessioned2025-08-21T15:25:47Z-
Data de disponibilização: dc.date.available2025-08-21T15:25:47Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2006-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.2353/ajpath.2006.050658-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/231011-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/231011-
Descrição: dc.descriptionWe have investigated the specific contribution of protease-activated receptor-2 (PAR2) to host defense during Porphyromonas gingivalis infection. Culture supernatants from P. gingivalis strains 33277 and W50 provoked Ca2+ mobilization in cells transfected with PAR2 (PAR2-KNRK) and desensitized the subsequent responses to PAR 2-selective agonist. In addition, culture supernatants of P. gingivalis E8 (RgpA/RgpB double knockout) did not cause calcium response in PAR2-KNRK cells, evidencing the involvement of the arginine-specific cysteine proteases RgpA and RgpB in PAR2 activation by P. gingivalis. Injection of P. gingivalis into mouse subcutaneous chambers provoked an increased proteolytic activity, which was inhibited by serine protease inhibitors. Fluids collected from chambers of P. gingivalis-injected mice were able to activate PAR2 and this activation was inhibited by serine protease inhibitors. P. gingivalis inoculation into subcutaneous chambers of wild-type mice induced an inflammatory response that was inhibited by a serine protease inhibitor and was significantly reduced in PAR2-deficient mice. Finally, mice orally challenged with P. gingivalis developed alveolar bone loss, which was significantly reduced in PAR2-deficient mice at 42 and 60 days after P. gingivalis infection. We conclude that PAR2 is activated on P. gingivalis infection, in which it plays an important role in the host inflammatory response. Copyright © American Society for Investigative Pathology.-
Descrição: dc.descriptionDepartment of Pharmacology and Therapeutics Faculty of Medicine University of Calgary, Calgary, Alta.-
Descrição: dc.descriptionCanadian Institute for Health Research Group in Matrix Dynamics Dental Research Institute University of Toronto, Toronto, Ont.-
Descrição: dc.descriptionDepartment of Periodontology and Oral Pathology Dental School of Araraquara State University of São Paulo, Araraquara, São Paulo-
Descrição: dc.descriptionDepartment of Dermatology Ludwig Boltzman Institute for Immunobiology of the Skin University Hospital, Muenster-
Descrição: dc.descriptionDrug Discovery Johnson and Johnson Pharmaceutical Research and Development, Spring House, PA-
Descrição: dc.descriptionPharmacology and Therapeutics Faculty of Medicine University of Calgary, 3330 Hospital Dr., NW, Calgary, Alta. T2N 4N1-
Formato: dc.format1189-1199-
Idioma: dc.languageen-
Relação: dc.relationAmerican Journal of Pathology-
???dc.source???: dc.sourceScopus-
Título: dc.titleProtease-activated receptor-2 activation: A major role in the pathogenesis of Porphyromonas gingivalis infection-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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