In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorLima, Lu M.-
Autor(es): dc.creatorMotisuki, C.-
Autor(es): dc.creatorPalomari Spolidorio, D. Madalena-
Autor(es): dc.creatorSantos Pinto, L.-
Data de aceite: dc.date.accessioned2025-08-21T18:17:26Z-
Data de disponibilização: dc.date.available2025-08-21T18:17:26Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2005-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/sj.ejcn.1602158-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/230989-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/230989-
Descrição: dc.descriptionObjective: This in vitro study compared the adhesion of two probiotics microorganisms (Lactobacillus casei Shirota and Lactobacillus acidophilus) to an artificial caries model. Method: In total, 30 bovine teeth were longitudinally sectioned, excluding the lingual half surface. The specimens were covered with nail varnish, except for an area of 3 by 5 mm in dentin, suspended in an artificial caries solution promoted by Streptococcus mutans, and incubated at 37°C. After 14 days, the specimens were separated equally into two groups and transferred to a brain heart infusion culture media containing L. acidophilus (group A) and L. casei Shirota (group S), at 37°C. After 48 h, the exposed area of dentin was washed with 1 ml of distilled water and the caries dentin was removed and dispersed in 1 ml of saline solution. The samples of distilled water and caries dentin were diluted and inoculated in Rogosa selective Lactobacillus agar. The results in CFU/ml were analysed by the Mann-Whitney test. Results: There was no significant difference between Groups A and S for the lactobacilli count in samples of distilled water (P=0.237). Conclusions: The amount of L. acidophilus in the artificially caries dentin was significantly superior compared to L. casei Shirota (P=0.047), suggesting an inferior adhesion potential for this microorganism. © 2005 Nature Publishing Group. All rights reserved.-
Descrição: dc.descriptionDepartment of Pediatric Dentistry Araraquara Dental School University of São Paulo State, Rua Humaitá 1680, 305 Araraquara-
Formato: dc.format884-886-
Idioma: dc.languageen-
Relação: dc.relationEuropean Journal of Clinical Nutrition-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCultured milk products-
Palavras-chave: dc.subjectDental caries-
Palavras-chave: dc.subjectLactobacillus acidophilus-
Palavras-chave: dc.subjectLactobacillus casei-
Título: dc.titleIn vitro evaluation of probiotics microorganisms adhesion to an artificial caries model-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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