Immunohistochemical expression of p16INK4a and bcl-2 according to HPV type and to the progression of cervical squamous intraepithelial lesions

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorGuimarães, Márcia C.M.-
Autor(es): dc.creatorGonçalves, Maria Alice G.-
Autor(es): dc.creatorSoares, Christiane P.-
Autor(es): dc.creatorBettini, Jussara S.R.-
Autor(es): dc.creatorDuarte, Roberta A.-
Autor(es): dc.creatorSoares, Edson G.-
Data de aceite: dc.date.accessioned2025-08-21T19:00:20Z-
Data de disponibilização: dc.date.available2025-08-21T19:00:20Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2005-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1369/jhc.4A6312.2005-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/230975-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/230975-
Descrição: dc.descriptionInactivation of the cell cycle inhibitor gene p16MTS1 seems to be involved in human papillomavirus (HPV)-related carcinogenesis because E6 and E7 oncoproteins may impair p16INK4a and, indirectly, bcl-2 functions. In this study, we analyzed the role of immunohistochemical expression of p16 INK4a and bcl-2 in HPV-infected cervical biopsies as prognostic markers of the progression of squamous intraepithelial lesion (SIL). Sixty-five cervical biopsies were stratified into two subgroups according to the second biopsy: 27 of them maintained a low-grade (LG)-SIL diagnosis, and 38 progressed from LG-SIL to high-grade (HG)-SIL. p16INK4a and bcl-2 quantitative expression levels were measured by the immunoperoxidase method. PCR-DNA techniques were used to detect and type HPV. The Wilcoxon and Fisher exact tests were employed for the statistical analysis. In the group with an LG-SIL diagnosis at the second biopsy, no significant associations were found between p16INK4a and bcl-2 expression and presence of HPV16/18. In the group that progressed to HG-SIL, a significant association was observed between p16INK4a overexpression and HPV16/18 presence (p = 0.021), but none with bcl-2 levels. It is concluded that immunohistochemical bcl-2 expression may not be useful for predicting the progression of HPV-related SIL. In contrast, p16INK4a overexpression seemed to be associated with HPV 16 and 18, suggesting that it may be a good marker for predicting SIL progression.-
Descrição: dc.descriptionDepartment of Pathology School of Medicine of Ribeirao Preto University of São Paulo, São Paulo-
Descrição: dc.descriptionDivision of Clinical Immunology School of Medicine of Ribeirao Preto University of São Paulo, São Paulo-
Descrição: dc.descriptionDepartment of Clinical Analyses Sch. Pharmaceutical Sci. Araraquara University of São Paulo State, Araraquara-
Descrição: dc.descriptionDepartment of Pathology School of Medicine of Ribeirao Preto University of São Paulo, Av. Bandeirantes, 3900, 14049-900 Ribeirão Preto, SP-
Formato: dc.format509-516-
Idioma: dc.languageen-
Relação: dc.relationJournal of Histochemistry and Cytochemistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBcl-2-
Palavras-chave: dc.subjectCarcinogenesis-
Palavras-chave: dc.subjectHuman papillomavirus-
Palavras-chave: dc.subjectp16INK4a-
Palavras-chave: dc.subjectSquamous intraepithelial lesions-
Título: dc.titleImmunohistochemical expression of p16INK4a and bcl-2 according to HPV type and to the progression of cervical squamous intraepithelial lesions-
Tipo de arquivo: dc.typelivro digital-
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