Assessment of monocytic component in acute myelomonocytic and monocytic/monoblastic leukemias by a chemiluminescent assay

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniv. S. Paulo at R. Preto Med. Sch.-
Autor(es): dc.creatorda Fonseca, Luiz M.-
Autor(es): dc.creatorBrunetti, Iguatemy L.-
Autor(es): dc.creatorCampa, Ana-
Autor(es): dc.creatorCatalani, Luiz H.-
Autor(es): dc.creatorCalado, Rodrigo T.-
Autor(es): dc.creatorFalcão, Roberto Passetto-
Data de aceite: dc.date.accessioned2025-08-21T19:36:41Z-
Data de disponibilização: dc.date.available2025-08-21T19:36:41Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2003-08-11-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/sj.thj.6200209-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/230932-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/230932-
Descrição: dc.descriptionIntroduction: Classically, the monocytic component of acute myelomonocytic (FAB-M4) and acute monocytic/monoblastic (FAB-M5) leukemias is demonstrated by nonspecific esterase positivity in cytochemical stainings. We have previously demonstrated that nonspecific esterases from normal monocytes can be determined by a chemiluminescent method. In the present study, we investigated whether this assay can also determine the monocytic component of FAB-M4 and FAB-M5 and distinguish these acute myeloid leukemia (AML) categories. Materials and Methods: Bone marrow samples were obtained from 66 patients with AML (M0, two cases; M1, 12 cases; M2, 13 cases; M3, 10 cases; M4, 11 cases; M5, 12 cases; M6, two cases; M7, four cases). Cells were incubated with a standard reaction mixture and chemiluminescence was measured for 10min. Two parameters were assessed, the peak (PLE) and the integrated light emission (ILE). Results: Both PLE and ILE were higher in FAB-M4 and FAB-M5 subtypes compared to other AML subtypes (P < 0.001). In addition, the classification of AML cases into FAB-M4, FAB-M5 and nonmonocytic subtypes based on ILE analysis was concordant with α-naphthyl acetate esterase (ANAE) in 97% of cases (kappa coefficient 0.94, P < 0.001). Conclusions: These findings indicate that this chemiluminescent assay was able to determine the monocytic component of FAB-M4 and FAB-M5 cells, and the classification of AML subtypes based on chemiluminescent analysis strongly agreed with the cytochemical ANAE-staining. In conclusion, this chemiluminescent assay is a simple, fast and objective method, which may be useful as an alternative tool in the differential diagnosis of AML subtypes.-
Descrição: dc.descriptionDepartment of Clinical Analysis State Univ. of São Paulo School of Pharmaceutical Science, Araraquara-
Descrição: dc.descriptionDepartment of Biochemistry Univ. of Sao Paulo Chem. Inst., São Paulo-
Descrição: dc.descriptionCenter for Cell-based Therapy Department of Clinical Medicine Univ. S. Paulo at R. Preto Med. Sch., Av. Bandeirantes 3900, Ribeirão Preto, 14049-900 SP-
Formato: dc.format26-30-
Idioma: dc.languageen-
Relação: dc.relationHematology Journal-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAcute myeloid leukemia-
Palavras-chave: dc.subjectChemiluminescence-
Palavras-chave: dc.subjectDiagnosis-
Palavras-chave: dc.subjectEsterase-
Palavras-chave: dc.subjectMonocyte-
Título: dc.titleAssessment of monocytic component in acute myelomonocytic and monocytic/monoblastic leukemias by a chemiluminescent assay-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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