Produção e caracterização de α-amilase produzida por Rhizopus sp.

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorFaculdade de Ciências e Letras-
Autor(es): dc.creatorDe Souza, Elisângela Lucio-
Autor(es): dc.creatorHoffmann, Erika H.E.-
Autor(es): dc.creatorCastilho, Vanessa M.-
Autor(es): dc.creatorDe Lima, Viviane A.-
Autor(es): dc.creatorBellini, Márcia Z.-
Autor(es): dc.creatorVinícius D'Arcadia Cruz, M.-
Autor(es): dc.creatorCruz, Rubens-
Data de aceite: dc.date.accessioned2025-08-21T20:03:39Z-
Data de disponibilização: dc.date.available2025-08-21T20:03:39Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued1996-12-01-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/230711-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/230711-
Descrição: dc.descriptionA strain of Rhizopus sp. screened among more than 800 filamentous fungi showed great ability to produce a thermostable α-amylase by solid state fermentation. The best production was obtained with a bran moisture content of 40% when the enzyme activity reached 60 EU/g. of medium. During the purification procedures, a column of DEAE-Sephadex A-50 separated the enzyme in two fractions and the larger (85% of the total activity) showed optimum pH in a range from -4.0 to 5.6. Optimum temperature was found at 60-65° C and in this range no loss of activity was observed after 60 min. of treatment in pH 5,0. Its Km and Vm are, respectively, of 5.0 mg/ml of starch and 10,01 uMol of reducing sugar/min./mg. of protein. Its molecular weight was calculated in 64.000 by gel filtration in Sepbadex G-200. The dextrinization power of the enzyme was observed preferentialy on substrates compound by chains with higher ramifications, that is: amylopectin > starch > amylose. Other aspects of the enzyme pattern action are also discussed.-
Descrição: dc.descriptionDepartamento de Ciências Biológicas Faculdade de Ciências e Letras Campus Universitário, Av. Dom Antonio, 2100, CEP 19.800-000 - Assis - SP-
Formato: dc.format831-839-
Idioma: dc.languagept_BR-
Relação: dc.relationArquivos de Biologia e Technologia-
???dc.source???: dc.sourceScopus-
Título: dc.titleProdução e caracterização de α-amilase produzida por Rhizopus sp.-
Título: dc.titleProduction and characterization of α-amilase from Rhizopus sp.-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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