Effect of Bacterial Infection on Ghrelin Receptor Regulation in Periodontal Cells and Tissues

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Autor(es): dc.contributorUniversity Medical Center of the Johannes Gutenberg University-
Autor(es): dc.contributorUniversity of Bonn-
Autor(es): dc.contributorUniversity of Bern-
Autor(es): dc.contributorUniversity Medical Centre of Regensburg-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorNogueira, Andressa V. B.-
Autor(es): dc.creatorNokhbehsaim, Marjan-
Autor(es): dc.creatorDamanaki, Anna-
Autor(es): dc.creatorEick, Sigrun-
Autor(es): dc.creatorBeisel-Memmert, Svenja-
Autor(es): dc.creatorKirschneck, Christian-
Autor(es): dc.creatorSchröder, Agnes-
Autor(es): dc.creatorCirelli, Thamiris-
Autor(es): dc.creatorLeguizamón, Natalia D. P.-
Autor(es): dc.creatorCirelli, Joni A.-
Autor(es): dc.creatorDeschner, James-
Data de aceite: dc.date.accessioned2025-08-21T18:35:31Z-
Data de disponibilização: dc.date.available2025-08-21T18:35:31Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ijms23063039-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/230571-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/230571-
Descrição: dc.descriptionThe effect of bacterial infection on the expression of growth hormone secretagogue receptor (GHS-R) was investigated in periodontal cells and tissues, and the actions of ghrelin were evaluated. GHS-R was assessed in periodontal tissues of rats with and without periodontitis. Human gingival fibroblasts (HGFs) were exposed to Fusobacterium nucleatum in the presence and absence of ghrelin. GHS-R expression was determined by real-time PCR and immunocytochemistry. Furthermore, wound healing, cell viability, proliferation, and migration were evaluated. GHS-R expression was significantly higher at periodontitis sites as compared to healthy sites in rat tissues. F. nucleatum significantly increased the GHS-R expression and protein level in HGFs. Moreover, ghrelin significantly abrogated the stimulatory effects of F. nucleatum on CCL2 and IL-6 expressions in HGFs and did not affect cell viability and proliferation significantly. Ghrelin stimulated while F. nucleatum decreased wound closure, probably due to reduced cell migration. Our results show original evidence that bacterial infection upregulates GHS-R in rat periodontal tissues and HGFs. Moreover, our study shows that ghrelin inhibited the proinflammatory actions of F. nucleatum on HGFs without interfering with cell viability and proliferation, suggesting that ghrelin and its receptor may act as a protective molecule during bacterial infection on periodontal cells.-
Descrição: dc.descriptionDeutscher Akademischer Austauschdienst-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartment of Periodontology and Operative Dentistry University Medical Center of the Johannes Gutenberg University-
Descrição: dc.descriptionSection of Experimental Dento-Maxillo-Facial Medicine Center of Dento-Maxillo-Facial Medicine University of Bonn-
Descrição: dc.descriptionLaboratory of Oral Microbiology Department of Periodontology University of Bern-
Descrição: dc.descriptionDepartment of Orthodontics Center of Dento-Maxillo-Facial Medicine University of Bonn-
Descrição: dc.descriptionDepartment of Orthodontics University Medical Centre of Regensburg-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University-UNESP, SP-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University-UNESP, SP-
Descrição: dc.descriptionDeutscher Akademischer Austauschdienst: 57391253-
Descrição: dc.descriptionCAPES: 88881.144012/2017-01-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Molecular Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectFusobacterium nucleatum-
Palavras-chave: dc.subjectGhrelin receptor-
Palavras-chave: dc.subjectHuman gingival fibroblast-
Palavras-chave: dc.subjectPeriodontal tissues-
Palavras-chave: dc.subjectPeriodontitis-
Título: dc.titleEffect of Bacterial Infection on Ghrelin Receptor Regulation in Periodontal Cells and Tissues-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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