A novel peptide-based electrochemical biosensor for breast cancer characterization over a poly 3-(3-aminophenyl) propionic acid matrix

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de Uberlândia (UFU)-
Autor(es): dc.creatorda Fonseca Alves, Rafael-
Autor(es): dc.creatorMartins, Isabella Castro-
Autor(es): dc.creatorFranco, Diego Leoni-
Autor(es): dc.creatorSilva, Adriane das Graças-
Autor(es): dc.creatorde Souza Santos, Paula-
Autor(es): dc.creatorGoulart, Luiz Ricardo-
Autor(es): dc.creatorCristina de Paiva Maia, Yara-
Autor(es): dc.creatorFaria Silva, Alinne Tatiane-
Autor(es): dc.creatorGonçalves Araújo, Thaise-
Autor(es): dc.creatorDel Pilar Taboada Sotomayor, Maria-
Data de aceite: dc.date.accessioned2025-08-21T17:21:47Z-
Data de disponibilização: dc.date.available2025-08-21T17:21:47Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.bios.2022.114081-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/230555-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/230555-
Descrição: dc.descriptionIn this work, a new electrochemical biosensor was developed using peptides selected by Phage Display as biorecognition phase to Breast Cancer (BC) characterization. Phage clones were selected against MCF-7 (ER-positive BC) proteins, in order to characterize patients with aggressive luminal BC. Biotin-C3 and biotin-H2 peptides were chemically synthesized and validated by flow cytometry, immunofluorescence assays, and ELISA assays, being more reactive to the MCF-7 lineage. Furthermore, a new matrix for the coupling of biomolecules on the surface of graphite electrodes was generated, through electrochemical modification with a new material derived from 3-(3-aminophenyl)propionic acid (3-3-APPA). Electrochemical and morphological characterizations were carried out, and the mechanism of electropolymerization of poly(3-3-APPA) was proposed, in which the carboxylate groups are kept in the structure of the formed polymer. Then, a biosensor was developed by immobilizing the biotin-C3 and biotin-H2 peptides in the SPE/poly(3-3-APPA)/avidin system for the detection of BC tumor markers in serological samples. Finally, peptides were validated using samples from patients with BC and Benign Breast Disease. Biotin-C3 peptide characterized luminal BC according to p53 status and to HER2 expression, being the biosensor a better strategy when compared to ELISA test. This new biosensor will open a new perspective for a rapid and electrochemical platform for the characterization of BC and its molecular subtypes.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)-
Descrição: dc.descriptionDepartment of Analytical Chemistry Institute of Chemistry State University of São Paulo (UNESP), SP-
Descrição: dc.descriptionInstitute of Biotechnology (IBTEC) Federal University of Uberlandia (UFU), MG-
Descrição: dc.descriptionInstitute of Chemistry Federal University of Uberlandia (UFU), MG-
Descrição: dc.descriptionFaculty of Medicine (FAMED) Federal University of Uberlandia (UFU), MG-
Descrição: dc.descriptionDepartment of Analytical Chemistry Institute of Chemistry State University of São Paulo (UNESP), SP-
Descrição: dc.descriptionFAPEMIG: 2019/11776-
Descrição: dc.descriptionFAPEMIG: APQ-00760-18-
Idioma: dc.languageen-
Relação: dc.relationBiosensors and Bioelectronics-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject3-(3-Aminophenyl)propionic acid-
Palavras-chave: dc.subjectBiosensors-
Palavras-chave: dc.subjectBreast cancer-
Palavras-chave: dc.subjectModified electrodes-
Palavras-chave: dc.subjectPhage display-
Título: dc.titleA novel peptide-based electrochemical biosensor for breast cancer characterization over a poly 3-(3-aminophenyl) propionic acid matrix-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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