The use of differential separation and density gradient with AllGrad 90% after thawing improves the sperm quality of South American catfish (Rhamdia quelen)

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorAnimal Science Research Program of Federal University of Rio Grande do Sul-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Vale of Taquari - Univates-
Autor(es): dc.contributorProgram of Federal University of Rio Grande do Sul-
Autor(es): dc.contributorCatholic University of Temuco-
Autor(es): dc.creatorPérez-Atehortúa, Maritza-
Autor(es): dc.creatorGiannotti Galuppo, Andrea-
Autor(es): dc.creatorBatista Rodrigues, Rômulo-
Autor(es): dc.creatorde Souza França, Thales-
Autor(es): dc.creatordos Santos Teixeira, Nathalia-
Autor(es): dc.creatorRodrigues de Freitas, Thaiza-
Autor(es): dc.creatorSantos Marques, Lis-
Autor(es): dc.creatorCossina Gomes, Itamar-
Autor(es): dc.creatorLisbôa Benato, Jhony-
Autor(es): dc.creatorFlores, Thales-
Autor(es): dc.creatorSanches, Eduardo Antônio-
Autor(es): dc.creatorSeabra de Souza, Ana Regina-
Autor(es): dc.creatorBustamante, Ivan-
Autor(es): dc.creatorQuirino, Monike-
Autor(es): dc.creatorPandolfo Bortolozzo, Fernando-
Autor(es): dc.creatorStreit Jr., Danilo Pedro-
Data de aceite: dc.date.accessioned2025-08-21T18:11:35Z-
Data de disponibilização: dc.date.available2025-08-21T18:11:35Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-05-15-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.aquaculture.2022.738072-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/230496-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/230496-
Descrição: dc.descriptionThe use of technologies in assisted reproduction, such as the use of centrifugation, has been reported as a tool that allows separating the best portion of sperm from cryopreserved milt. The main of this work was to compare two separation techniques, differential and density gradient, on thawed Rhamdia quelen milt, based on sperm functionality and fertilization capacity. Three treatments were compared: control, differential centrifugation or pelletization (T1), and density gradient centrifugation (T2). Motility percentage was better for differential (42.58 ± 6.50%) than for the control treatment (32.44 ± 6.03%). Membrane integrity and kinetic parameters was not different between treatments. The presence of normal sperm was higher in T2 treatment (13.55 ± 5.82%) when compared to the control (10.72 ± 3.53%) and T1 (3.97 ± 1.95%). There was a lower rate of morphological tail damage in the T1 (26.91 ± 7.13%) treatment compared to the T2 (37.48 ± 10.81%) and control (45.88 ± 4.84%). For the head morphologies, the lowest percentages were for the control (35.72 ± 5.89%) and T2 (4.89 ± 14.94%) treatments compared to the T1 treatment (62.59 ± 9.67%). In the MTT (3- (4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) test (AU/100 million sptz), the centrifuged treatments resulted in values of 1.58 ± 0.79 (T1) and 2.02 ± 1.88 (T2), better than the treatment control (0.20 ± 0.08). Likewise, the fertilization and hatching rates, respectly, were better for the T1 (76.68 ± 15.22% and 54.62 ± 17.39%) and T2 (69.85 ± 17.85% and 50.87 ± 20.59%) treatments, than the control treatment (40.03 ± 11.67% and 24.14 ± 13.97%). The comparison of differential centrifugation with density gradient centrifugation was carried out for the first time with cryopreserved milt of R. quelen. In summary, both centrifugation techniques proved to be efficient in obtaining a better portion of functional sperm from thawed milt. This is based on the high rates of embryonic survival and hatching obtained after both differential and density gradient separation.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionAnimal Science Research Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 7712, RS-
Descrição: dc.descriptionAquaculture Center Fisheries Engineering Department Paulista State University Júlio de Mesquita Filho, SP-
Descrição: dc.descriptionBiotechnology Laboratory University of Vale of Taquari - Univates, Avelio Tallini Street, 171, RS-
Descrição: dc.descriptionFaculty of Veterinary Medicine Swine Sector Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 9090, RS-
Descrição: dc.descriptionDepartment of Agricultural and Aquacultural Sciences Faculty of Natural Resources Catholic University of Temuco-
Descrição: dc.descriptionAquaculture Center Fisheries Engineering Department Paulista State University Júlio de Mesquita Filho, SP-
Descrição: dc.descriptionCAPES: CAPES/PROEX N° 88882.346090/2015-01-
Idioma: dc.languageen-
Relação: dc.relationAquaculture-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCentrifugation-
Palavras-chave: dc.subjectCryopreservation-
Palavras-chave: dc.subjectFish-
Palavras-chave: dc.subjectHatch-
Palavras-chave: dc.subjectMorphology-
Palavras-chave: dc.subjectSpawning-
Título: dc.titleThe use of differential separation and density gradient with AllGrad 90% after thawing improves the sperm quality of South American catfish (Rhamdia quelen)-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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