Antifungal Activity and Biocompatibility of α-AgVO3, α-Ag2WO4, and β-Ag2MoO4 Using a Three-Dimensional Coculture Model of the Oral Mucosa

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorPimentel, Bruna Natália Alves da Silva-
Autor(es): dc.creatorMarin-Dett, Freddy Humberto-
Autor(es): dc.creatorAssis, Marcelo-
Autor(es): dc.creatorBarbugli, Paula Aboud-
Autor(es): dc.creatorLongo, Elson-
Autor(es): dc.creatorVergani, Carlos Eduardo-
Data de aceite: dc.date.accessioned2025-08-21T18:01:48Z-
Data de disponibilização: dc.date.available2025-08-21T18:01:48Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-02-13-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fbioe.2022.826123-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/230465-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/230465-
Descrição: dc.descriptionFungal infections have become a major concern in the medical community, especially those caused by Candida spp. Within this species, Candida albicans stands out for being an opportunistic commensal fungus that can cause superficial and invasive infections. Current antifungal therapy involves the local and/or systemic use of drugs such as azoles, polyenes, and echinocandins. These antifungals are based on highly specific target sites, and the development of resistance may occur with changes in the enzymatic pathways that serve as the drug targets. Thus, the development of new antifungal drugs is highly recommended to prevent drug resistance. The present investigation evaluated the antifungal activity of silver-containing microcrystals such as silver vanadate (α-AgVO3), silver tungstate (α-Ag2WO4), and silver molybdate (β-Ag2MoO4). In addition to having antimicrobial activity, such compounds should not cause damage to underlying tissues. Thus, to better assess the biocompatibility of new compounds, a new three-dimensional (3D) coculture model involving three cell lines was developed. The validation of the model was based on fluorescent markers and confocal laser microscopy. The biocompatibility of silver-containing microcrystals was evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. 3D coculture was infected with C. albicans biofilm and challenged with α-AgVO3, α-Ag2WO4, and β-Ag2MoO4. The action of microcrystals on C. albicans biofilm was evaluated by colony-forming units (CFU/ml) and LIVE/DEAD staining. In addition, production of proinflammatory cytokines interleukin 6 (IL-6), IL-8, IL-1β, and tumor necrosis factor α (TNF-α) was measured by cytometric bead array kit using flow cytometry. The 3D coculture model described here proved to be adequate to assess both the biocompatibility of the new materials and the infectious processes. Regarding the biocompatibility of the microcrystals, only α-AgVO3 (15.62 µg/ml) showed a decrease in cell viability. The antibiofilm activity of α-Ag2WO4 was similar to that of the standard drug (fluconazole). Although α-Ag2WO4 was able to induce the production of IL-6, IL-8, and IL-1β, no differences in cytokine production were observed between noninfected and infected models treated with this microcrystal. β-Ag2MoO4 inhibits the production of TNF-α in the infected model; however, it showed no antibiofilm activity. Based on the biocompatibility and antifungal findings, α-Ag2WO4 is a promising material for treating C. albicans infection.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUniversidade Estadual Paulista-
Descrição: dc.descriptionLaboratory of Applied Microbiology Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Clinical Analysis School of Pharmaceutical Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionCDMF LIEC Chemistry Department Federal University of São Carlos (UFSCar)-
Descrição: dc.descriptionLaboratory of Applied Microbiology Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Clinical Analysis School of Pharmaceutical Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2013/07296-2 2018/01677-4-
Descrição: dc.descriptionCNPq: 431895/2016-3-
Descrição: dc.descriptionUniversidade Estadual Paulista: 88887.335189/2019-00-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Bioengineering and Biotechnology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject3D cultures-
Palavras-chave: dc.subjectCandida albicans-
Palavras-chave: dc.subjectcytokines-
Palavras-chave: dc.subjectinfection-
Palavras-chave: dc.subjectmicrocrystals-
Palavras-chave: dc.subjectsilver-
Título: dc.titleAntifungal Activity and Biocompatibility of α-AgVO3, α-Ag2WO4, and β-Ag2MoO4 Using a Three-Dimensional Coculture Model of the Oral Mucosa-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.