DNase enhances photodynamic therapy against fluconazole-resistant Candida albicans biofilms

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorAbreu-Pereira, César Augusto-
Autor(es): dc.creatorKlein, Marlise Inêz-
Autor(es): dc.creatorLobo, Carmélia Isabel Vitorino-
Autor(es): dc.creatorGorayb Pereira, Ana Luiza-
Autor(es): dc.creatorJordão, Cláudia Carolina-
Autor(es): dc.creatorPavarina, Ana Claudia-
Data de aceite: dc.date.accessioned2025-08-21T20:09:54Z-
Data de disponibilização: dc.date.available2025-08-21T20:09:54Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/odi.14149-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/230408-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/230408-
Descrição: dc.descriptionObjective: This study evaluated the effectiveness of DNase I combined with antimicrobial photodynamic therapy, mediated by Photodithazine® and light-emitting diode light, against biofilms formed by a fluconazole-resistant Candida albicans strain (ATCC 96901) and two clinical isolates (R14 and R70). Materials and Methods: Biofilms were grown for 48 h and exposed to DNase for 5 min, followed by application of a photosensitizer (P) and light (L), either singly or combined (P+L+, P−L+, P+L−, P−L−, P−L−DNase, P+L+DNase, P+L−DNase, and P−L+DNase; n = 12). Biofilm analysis included quantification of extracellular matrix components (water-soluble and insoluble polysaccharides, proteins and extracellular DNA), and biomass (total and insoluble), as well as the enumeration of colony-forming units. The data were analyzed using three-way analysis of variance with Bonferroni's post hoc test. Results: The DNase treatment combined with aPDT showed a reduction of 1.92, 1.65, and 1.29 log10 of cell viability compared with untreated controls for ATCC 96901, R14, and R70 strains, respectively. It also reduced extracellular matrix contents of water-soluble polysaccharides (36.3%) and extracellular DNA (72.3%), as well as insoluble biomass content (43.3%). Conclusion: The three strains showed similar behavior when treated with DNase, and the extracellular matrix components were affected, improving the effectiveness of antimicrobial photodynamic therapy.-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry at Araraquara Univ Estadual Paulista - UNESP-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry at Araraquara Univ Estadual Paulista - UNESP-
Idioma: dc.languageen-
Relação: dc.relationOral Diseases-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectantifungal drug resistance-
Palavras-chave: dc.subjectCandida albicans-
Palavras-chave: dc.subjectPhotochemotherapy-
Título: dc.titleDNase enhances photodynamic therapy against fluconazole-resistant Candida albicans biofilms-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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