Methylated DNA impedimetric immunosensor based on azo-polymer-AuNPs dots and 5-methylcytosine antibody using dissolved oxygen as a redox probe

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorOlean-Oliveira, André-
Autor(es): dc.creatorMonteiro Seraphim, Patricia-
Autor(es): dc.creatorTeixeira, Marcos F.S.-
Data de aceite: dc.date.accessioned2025-08-21T16:49:42Z-
Data de disponibilização: dc.date.available2025-08-21T16:49:42Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.elecom.2022.107242-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/230377-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/230377-
Descrição: dc.descriptionThe present work describes the development of an electrochemical immunosensor for the direct determination of methylated DNA using dissolved oxygen as the redox probe. The oxygen-sensitive response is possible due to the presence of a redox conducting polymer based on the Bismarck Brown Y dye (poly(azo-BBY)). In addition to the azo-polymer, gold nanoparticles (AuNPs) were formed in the polymer film by encapsulation to increase the active surface area and enhance sensor performance. The platform was easily synthesized using a single-step electropolymerization technique in a solution containing the BBY monomer and HAuCl4 salt. The immunosensor was developed by simply immobilizing the 5-methylcytosine antibody (Ab-5-mC) with high affinity to 5-mC on the surface of a device coated with azo-polymer-AuNPs dots. No secondary antibodies or enzymes were used in constructing this device. The immunosensor performance was evaluated by electrochemical impedance spectroscopy (EIS) in different concentrations of 5-methylcytosine (5-mC) in 0.10 mol L−1 phosphate buffer solution (PBS) solution containing atmospheric oxygen. A calibration curve was obtained by varying the resistance of the system as a function of the 5-mC concentration, revealing a limit of detection (LOD) in picograms per milliliter.-
Descrição: dc.descriptionDepartment of Chemistry and Biochemistry – School of Science and Technology Sao Paulo State University (UNESP), Rua Roberto Simonsen, 305, CEP 19060-900 – Presidente Prudente-
Descrição: dc.descriptionDepartment of Physiotherapy – School of Science and Technology Sao Paulo State University (UNESP), Rua Roberto Simonsen, 305, CEP 19060-900 - Presidente Prudente-
Descrição: dc.descriptionDepartment of Chemistry and Biochemistry – School of Science and Technology Sao Paulo State University (UNESP), Rua Roberto Simonsen, 305, CEP 19060-900 – Presidente Prudente-
Descrição: dc.descriptionDepartment of Physiotherapy – School of Science and Technology Sao Paulo State University (UNESP), Rua Roberto Simonsen, 305, CEP 19060-900 - Presidente Prudente-
Idioma: dc.languageen-
Relação: dc.relationElectrochemistry Communications-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectGold nanoparticle-polymer-
Palavras-chave: dc.subjectImmunosensor-
Palavras-chave: dc.subjectMethylated DNA sensor-
Palavras-chave: dc.subjectMethylcytosine antibody-
Palavras-chave: dc.subjectSecondary antibody less-
Título: dc.titleMethylated DNA impedimetric immunosensor based on azo-polymer-AuNPs dots and 5-methylcytosine antibody using dissolved oxygen as a redox probe-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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