The proteomic landscape of ovarian cancer cells in response to melatonin

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorSão José do Rio Preto-
Autor(es): dc.contributorUniversidade Estadual do Norte do Paraná – UENP-
Autor(es): dc.contributorUT Health-
Autor(es): dc.creatorCesário, Roberta Carvalho-
Autor(es): dc.creatorGaiotte, Leticia Barbosa-
Autor(es): dc.creatorCucielo, Maira Smaniotto-
Autor(es): dc.creatorSilveira, Henrique Spaulonci-
Autor(es): dc.creatorDelazari dos Santos, Lucilene-
Autor(es): dc.creatorde Campos Zuccari, Debora Aparecida Pires-
Autor(es): dc.creatorSeiva, Fábio Rodrigues Ferreira-
Autor(es): dc.creatorReiter, Russel J.-
Autor(es): dc.creatorde Almeida Chuffa, Luiz Gustavo-
Data de aceite: dc.date.accessioned2025-08-21T20:58:00Z-
Data de disponibilização: dc.date.available2025-08-21T20:58:00Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.lfs.2022.120352-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/230333-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/230333-
Descrição: dc.descriptionOvarian cancer (OC) is the most lethal gynecological malignancy with a highly negative prognosis. Melatonin is an indoleamine secreted by the pineal gland during darkness and has shown antitumor activity in both in vitro and in vivo experiments. Herein, we investigated the influence of melatonin on the proteome of human ovarian carcinoma cells (SKOV-3 cell line) using the Ultimate 3000 LC Liquid NanoChromatography equipment coupled to a Q-Exactive mass spectrometry. After 48 h of treatment, melatonin induced a significant cytotoxicity especially with the highest melatonin concentration. The proteomic profile revealed 639 proteins in the control group, and 98, 110, and 128 proteins were altered by melatonin at the doses of 0.8, 1.6, and 2.4 mM, respectively. Proteins associated with the immune system and tricarboxylic acid cycle were increased in the three melatonin-exposed groups of cells. Specifically, the dose of 2.4 mM led to a reduction in molecules associated with protein synthesis, especially those of the ribosomal protein family. We also identified 28 potential genes shared between normal ovarian tissue and OC in all experimental groups, and melatonin was predicted to alter genes encoding ribosomal proteins. Notably, the set of proteins changed by melatonin was linked to a better prognosis for OC patients. We conclude that melatonin significantly alters the proteome of SKOV-3 cells by changing proteins involved with the immune response and mitochondrial metabolism. The concentration of 2.4 mM of melatonin promoted the largest number of protein changes. The evidence suggests that melatonin may be an effective therapeutic strategy against OC.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences UNESP - Sao Paulo State University-
Descrição: dc.descriptionBiotechnology Institute (IBTEC) São Paulo State University (UNESP)-
Descrição: dc.descriptionFaculty of Medicine of São José do Rio Preto São José do Rio Preto-
Descrição: dc.descriptionDepartment of Biology Biological Science Center Universidade Estadual do Norte do Paraná – UENP, Luiz Meneghel Campus-
Descrição: dc.descriptionDepartament of Cell Systems and Anatomy UT Health-
Descrição: dc.descriptionGraduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences UNESP - Sao Paulo State University-
Descrição: dc.descriptionBiotechnology Institute (IBTEC) São Paulo State University (UNESP)-
Descrição: dc.descriptionGraduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2019/00906-6-
Descrição: dc.descriptionCNPq: 304108/2020-0-
Descrição: dc.descriptionCAPES: 88887.482368/2020-00-
Idioma: dc.languageen-
Relação: dc.relationLife Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectImmune response-
Palavras-chave: dc.subjectMelatonin-
Palavras-chave: dc.subjectMitochondrial metabolism-
Palavras-chave: dc.subjectOvarian cancer-
Palavras-chave: dc.subjectProteome-
Palavras-chave: dc.subjectSKOV-3 cells-
Título: dc.titleThe proteomic landscape of ovarian cancer cells in response to melatonin-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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