Synthesis and characterization of gold nanoparticles and their toxicity in alternative methods to the use of mammals

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorThe University of Queensland-
Autor(es): dc.contributorUniversidade Federal de Santa Catarina (UFSC)-
Autor(es): dc.contributorUniversity of Caxias do Sul (UCS)-
Autor(es): dc.contributorFederal University of Southern and Southeastern Pará (UNIFESSPA)-
Autor(es): dc.creatorMarcelino, Monica Yonashiro-
Autor(es): dc.creatorBorges, Felipe Azevedo-
Autor(es): dc.creatorScorzoni, Liliana-
Autor(es): dc.creatorde Lacorte Singulani, Junya-
Autor(es): dc.creatorGarms, Bruna Cambraia-
Autor(es): dc.creatorNiemeyer, Júlia Carina-
Autor(es): dc.creatorGuerra, Nayrim Brizuela-
Autor(es): dc.creatorPegorin Brasil, Giovana Sant'Ana-
Autor(es): dc.creatorMussagy, Cassamo Ussemane-
Autor(es): dc.creatorde Oliveira Carvalho, Francisco Adriano-
Autor(es): dc.creatorScontri, Mateus-
Autor(es): dc.creatorMendes-Giannini, Maria José Soares-
Autor(es): dc.creatorFusco-Almeida, Ana Marisa-
Autor(es): dc.creatorHerculano, Rondinelli Donizetti-
Data de aceite: dc.date.accessioned2025-08-21T20:01:14Z-
Data de disponibilização: dc.date.available2025-08-21T20:01:14Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jece.2021.106779-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229898-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229898-
Descrição: dc.descriptionIn this work we synthesize and characterize AuNPs using two chemical routes: via sodium citrate and via sodium citrate/borohydride. The cytotoxicity of AuNPs was tested by traditional in vitro assays and the in vivo toxicity was evaluated using alternative animals (Galleria mellonella and Caenorhabditis elegans). Avoidance tests with Folsomia candida were carried out to evaluate possible toxic effects in the soil ecosystem. Through Scanning Electron Microscopy and Transmission Electron Microscopy we verified the spherical shape of the AuNPs, with diameters of 34.8 ± 5.5 nm (via sodium citrate) and 7.9 ± 2.2 nm (via sodium citrate/borohydride). The hydrodynamic diameters values were compatible with those obtained microscopically, 30.1 ± 10.1 nm (method A) and 8.3 ± 2.8 nm (method B). Stability of up to 6 months was verified for AuNPs synthesized via sodium citrate, while synthesis via citrate/sodium borohydride was stable for only 1 month. The AuNPs from method A showed better efficacy in the NPs formation with higher absorbance in the plasmonic band at 523 nm, higher concentration and size, spherical shape, homogeneity and stability. The cytotoxicity of the two AuNPs exhibited a slight dose- and size-dependent behavior, with cell viability greater than 80%. The in vivo toxicity in the G. mellonella model showed a survival rate of 100% for 7 days, however, with C. elegans, the percent of survival increased as the concentration of AuNPs decreased. All concentrations of AuNPs, except for the AuNPs synthesized by the citrate pathway (1 mg/kg), showed non-avoidance responses when exposed to contaminated soil.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences Department of Clinical Analysis São Paulo State University (UNESP)-
Descrição: dc.descriptionInstitute of Chemistry Department of Biochemistry & Chemical Technology São Paulo State University (UNESP)-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences Department of Bioprocesses and Biotechnology Engineering São Paulo State University (UNESP)-
Descrição: dc.descriptionSchool of Chemistry and Molecular Biosciences The University of Queensland-
Descrição: dc.descriptionPostgraduate Program in Agricultural and Natural Ecosystems (PPGEAN) Federal University of Santa Catarina (UFSC)-
Descrição: dc.descriptionArea of Exact Sciences and Engineering University of Caxias do Sul (UCS)-
Descrição: dc.descriptionInstitute of Exact Sciences Federal University of Southern and Southeastern Pará (UNIFESSPA)-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences Department of Clinical Analysis São Paulo State University (UNESP)-
Descrição: dc.descriptionInstitute of Chemistry Department of Biochemistry & Chemical Technology São Paulo State University (UNESP)-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences Department of Bioprocesses and Biotechnology Engineering São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2016/11836-0-
Descrição: dc.descriptionFAPESP: 2017/19603-8-
Descrição: dc.descriptionCNPq: 470261/2012-9-
Idioma: dc.languageen-
Relação: dc.relationJournal of Environmental Chemical Engineering-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAlternative animal model-
Palavras-chave: dc.subjectAvoidance response-
Palavras-chave: dc.subjectGold nanoparticles-
Palavras-chave: dc.subjectNanotoxicity-
Título: dc.titleSynthesis and characterization of gold nanoparticles and their toxicity in alternative methods to the use of mammals-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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